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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Helicobacter pylori [ICD11: XN3DY

]

NCBI PubMed ID: 18436591
Publication DOI: 10.1099/jmm.0.47783-0
Journal NLM ID: 0224131
Publisher: Reading, England: Society for General Microbiology
Correspondence: Eleonora.altman

nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada, Instituto de Microbiología Clínica, Edificio de Ciencias Biomédicas, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile, Instituto de Medicina, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile

Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le(x)) and Le(y) epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le(a) and Le(b) structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Le(x) and/or Le(y) epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Le(b) blood group determinants, in addition to type 2 Le(x) and Le(y) structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %).

Lipopolysaccharide, Helicobacter pylori, Helicobacter, Lewis x, ELISA, Chile, Helicobacter Infections, Lewis Blood-Group System

Structure type: oligomer
Location inside paper: p.586, fig.1
Aglycon: lipid A
Compound class: LOS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_136044,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_190606,IEDB_2189046,IEDB_2189047,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88

Methods: Western blotting, serological methods, electrophoresis, whole-cell indirect ELISA
Biological activity: serological data
Comments, role: Part of the LPS from H.pylori (see ID: 22809,22810).

Related record ID(s): 22809, 22810
NCBI Taxonomy refs (TaxIDs): 210
Show glycosyltransferases

There is only one chemically distinct structure:

[1*]OC[C@@H](O)[C@H]1O[C@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H]([C@H](O)CO[C@@H]3O[C@H](CO)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@H](O)[C@H]3O)O[C@@H]2O[C@H]2[C@@H](O)[C@H](O)[C@@H]([C@@H](O)CO)O[C@@H]2O[C@@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](O)C[C@](O)(C(=O)O)O[C@@H]3[C@H](O)CO)O[C@H]([C@@H](O)COP(=O)(O)OCCN)[C@H]2O)[C@@H](O[C@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

R1 = O-antigen (IDs 22809, 22810)
Warning = sub-repeat ending at aDGlcp: unknown unit count was assumed to be 3

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Helicobacter pylori [ICD11: XN3DY

]

NCBI PubMed ID: 18436591
Publication DOI: 10.1099/jmm.0.47783-0
Journal NLM ID: 0224131
Publisher: Reading, England: Society for General Microbiology
Correspondence: Eleonora.altman

Lipopolysaccharide, Helicobacter pylori, Helicobacter, Lewis x, ELISA, Chile, Helicobacter Infections, Lewis Blood-Group System

Structure type: oligomer
Location inside paper: p.586, fig.1
Trivial name: Exo type 2 Lewis x/y
Contained glycoepitopes: IEDB_130644,IEDB_130646,IEDB_130654,IEDB_130655,IEDB_130697,IEDB_135813,IEDB_136044,IEDB_136045,IEDB_137340,IEDB_137472,IEDB_137776,IEDB_140108,IEDB_140122,IEDB_141500,IEDB_141794,IEDB_141807,IEDB_142489,IEDB_143250,IEDB_144556,IEDB_144562,IEDB_145669,IEDB_147455,IEDB_149555,IEDB_149557,IEDB_149561,IEDB_150092,IEDB_150787,IEDB_150939,IEDB_150948,IEDB_151531,IEDB_152214,IEDB_153553,IEDB_158546,IEDB_158550,IEDB_174333,IEDB_190606,IEDB_2151203,IEDB_461719,IEDB_461720,IEDB_461721,IEDB_952752,SB_147,SB_154,SB_157,SB_165,SB_166,SB_173,SB_187,SB_195,SB_30,SB_34,SB_7,SB_86,SB_88

Methods: Western blotting, serological methods, electrophoresis, whole-cell indirect ELISA
Biological activity: serological data
Comments, role: Le-x polysaccharide capped with Le-x/y; part of the LPS from H.pylori (see ID: 22603,22810).

Related record ID(s): 22603, 22810
NCBI Taxonomy refs (TaxIDs): 210
Reference(s) to other database(s): GTC:G68930NU
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]5[C@@H](CO)O[C@@H](O)[C@H](NC(C)=O)[C@H]5O[C@@H]5O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]5O)[C@@H]4O)[C@H](NC(C)=O)[C@H]3O[C@@H]3O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]3O)[C@@H]2O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O[C@@H]4O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]4O)[C@H](O[C@@H]4O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]4O)[C@H]3NC(C)=O)[C@H]2O)[C@@H]1O[C@@H]1O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at bDGlcpN: unknown unit count was assumed to be 3
Warning = sub-repeat ending at bDGlcpN: unknown unit count was assumed to be 3
Warning = sub-repeat ending at bDGlcpN: unknown unit count was assumed to be 3

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Helicobacter pylori [ICD11: XN3DY

]

NCBI PubMed ID: 18436591
Publication DOI: 10.1099/jmm.0.47783-0
Journal NLM ID: 0224131
Publisher: Reading, England: Society for General Microbiology
Correspondence: Eleonora.altman

Lipopolysaccharide, Helicobacter pylori, Helicobacter, Lewis x, ELISA, Chile, Helicobacter Infections, Lewis Blood-Group System

Structure type: polymer biological repeating unit
Location inside paper: p.586, fig.1
Trivial name: Endo type 2 Lewis x
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130646,IEDB_130654,IEDB_130655,IEDB_130697,IEDB_135813,IEDB_136044,IEDB_136045,IEDB_137340,IEDB_137472,IEDB_137776,IEDB_140108,IEDB_140122,IEDB_141500,IEDB_141794,IEDB_141807,IEDB_142489,IEDB_144556,IEDB_144562,IEDB_145669,IEDB_147455,IEDB_149557,IEDB_150092,IEDB_150939,IEDB_151531,IEDB_152214,IEDB_158550,IEDB_174333,IEDB_190606,IEDB_2151203,IEDB_461720,IEDB_952752,SB_157,SB_165,SB_166,SB_173,SB_187,SB_195,SB_30,SB_7,SB_86,SB_88

Methods: Western blotting, serological methods, electrophoresis, whole-cell indirect ELISA
Biological activity: serological data
Comments, role: Part of the LPS from H.pylori (see ID: 22603,22809)

Related record ID(s): 22603, 22809
NCBI Taxonomy refs (TaxIDs): 210
Reference(s) to other database(s): GTC:G79959AY, GlycomeDB:34500
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CO)O[C@@H]([*])[C@H](NC(C)=O)[C@H]2O[C@@H]2O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]2O)O[C@H](CO)[C@@H]1O

SVG MW SMILES 3D mol Ionize