Herasimenka Y, Cescutti P, Sampaio Noguera CE, Ruggiero JR, Urbani R, Impallomeni G, Zanetti F, Campidelli S, Prato M, Rizzo R Macromolecular properties of cepacian in water and in dimethylsulfoxide Carbohydrate Research343(1) (2008)
81-89
NCBI PubMed ID:18028888 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: rizzorunits.it Institutions: Department of Biochemistry, Biophysics and Macromolecules Chemistry, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy
Cepacian is the exopolysaccharide produced by the majority of the so far investigated clinical strains of the Burkholderia cepacia complex. This is a group of nine closely related bacterial species that might cause serious lung infections in cystic fibrosis patients, in some cases leading to death. In this paper the aggregation ability and the conformational properties of cepacian chain were investigated to understand its role in biofilm formation. Viscosity and atomic force microscopy studies in water and in mixed (dimethylsulfoxide/water) solvent indicated the formation of double stranded molecular structures in aqueous solutions. Inter-residue short distances along cepacian chain were investigated by NOE NMR, which showed that two side chains of cepacian were not conformationally free due to strong interactions with the polymer backbone. These interactions were attributed to hydrogen bonding and contributed to structure rigidity
Methods: 1H NMR, NMR-2D, HPSEC, microscopy, intrinsic viscosity measurements 3D data: 3D data
Related record ID(s): 1207, 3154, 4338, 7433, 10142, 10158, 10790, 20223, 21560, 25927 NCBI Taxonomy refs (TaxIDs):87882 Reference(s) to other database(s): GTC:G16935RB, GlycomeDB:25352 Show glycosyltransferases
Micheli L, Uccelletti D, Palleschi C, Crescenzi V Isolation and characterisation of a ropy Lactobacillus strain producing the exopolysaccharide kefiran Applied Microbiology and Biotechnology53(1) (1999)
69-74
The structure was elucidated in this paper NCBI PubMed ID:10645627 Publication DOI:10.1007/s002530051616 Journal NLM ID:8406612 Publisher: Springer Correspondence: Crescenzi V <crescenziaxrma.uniromal.it> Institutions: Department of Chemistry, University of Rome ‘‘La Sapienza’’, Rome, Italy, Department of Developmental and Cell Biology, University of Rome ‘‘La Sapienza’’, Rome, Italy
A capsular-polysaccharide-producing strain, LM-17, was isolated from kefir grains and was identified as a slime-forming, rod-shaped Lactobacillus. According to 1H- and 13C-NMR spectral data, the exopolysaccharide produced by the isolated bacterial strain is identical to the glucogalactan extracted from kefir grains and therefore known as kefiran. The kefiran produced was characterised by means of viscosity, optical rotatory power, circular dichroism and IR spectral measurements. A batch procedure was set up for the culture and extraction of the exopolysaccharide in laboratory conditions, resulting in a yield of 2 g/l purified kefiran from the culture supernatant of the LM-17 strain.
Methods: 13C NMR, 1H NMR, FTIR, extraction, optical rotation measurement, CD, intrinsic viscosity measurements, precipitation Comments, role: extracts from kefir grain and from isolated strain are equal; -6)bDGlcp(1-?)[bDGlcp(1-?)]bDGalp means that bDGalp is substituted at 2 or 6 positions
Related record ID(s): 46650 NCBI Taxonomy refs (TaxIDs):1578 Reference(s) to other database(s): GTC:G91956TA Show glycosyltransferases
NMR conditions: in D2O at 353 K
1H NMR data: present in publication
13C NMR data: present in publication
There are 12 chemically distinct structures. Please, select:
Yuan QX, Zhao LY, Li ZH, Harqin C, Peng YF, Liu JK Physicochemical analysis, structural elucidation and bioactivities of a high-molecular-weight polysaccharide from Phellinus igniarius mycelia International Journal of Biological Macromolecules120(B) (2018)
1855-1864
The structure was elucidated in this paper NCBI PubMed ID:30287368 Publication DOI:10.1016/j.ijbiomac.2018.09.192 Journal NLM ID:7909578 Publisher: Butterworth-Heinemann Correspondence: Yuan QX <qingxiayuanmail.scuec.edu.cn>; Zhao LY <zhaolongyanmail.scuec.edu.cn>; Li ZH <2015051mail.scuec.edu.cn>; Liu JK <liujikaimail.scuec.edu.cn> Institutions: School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China
Phellinus igniarius is a well-known edible and medicinal fungus consumed in Asia for centuries. In the present study, a polysaccharide named PIP-1 was obtained from Phellinus igniarius mycelia, its physicochemical properties were determined, its detailed structures were elucidated by analysis of its depolymerized product, and its antioxidant and antitumor activities were evaluated. Results showed that PIP-1 had a high molecular weight, a high intrinsic viscosity and a linear repeating backbone composed of glucopyranose (Glcp), galactopyranose, and mannopyranose joined by α-(1→4), α-(1→3), and α-(1→6) linkages, and single α‑terminal‑D‑Glcp as side chains 6-O-linked to the main chain according to the GC-MS and NMR results, and exhibited triple helical structure. Pharmacological results revealed that PIP-1 could effectively scavenge hydroxyl radicals, partly scavenge DPPH radials and chelate ferrous metal ions. Furthermore, PIP-1 showed potent inhibitory effects on growth of HT-29 and MCF-7 cells. These findings suggested that high-molecular-weight PIP-1 containing triple helical structure and only α-type glycosidic bond holds promise as novel functional foods and agents to promote human health.
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(previously named: Pseudomonas cepacia complex)
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