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Structure type: monomer
Trivial name: cordycerebroside A, cerebroside A, cordycerebroside A
Compound class: glycolipid, cerebroside
Contained glycoepitopes: IEDB_137339,IEDB_142488,IEDB_146664,IEDB_983931,SB_192,SB_5

• Article ID: 9371
  Chiu C-P, Liu S-C, Tang C-H, Chan Y, El-Shazly M, Lee C-L, Du Y-C, Wu T-Y, Chang F-R, Wu Y-C "Anti-inflammatory cerebrosides from cultivated Cordyceps militaris" - Journal of Agricultural and Food Chemistry 64(7) (2016) 1540-1548
  

  Cordyceps militaris (bei-chong-chaw, northern worm grass) is a precious and edible entomopathogenic fungus, which is widely used in traditional Chinese medicine (TCM) as a general booster for the nervous system, metabolism, and immunity. Saccharides, nucleosides, mannitol, and sterols were isolated from this fungus. The biological activity of C. militaris was attributed to the saccharide and nucleoside contents. In this study, the aqueous methanolic fraction of C. militaris fruiting bodies exhibited a significant anti-inflammatory activity. Bioactivity-guided fractionation of the active fraction led to the isolation of eight compounds, including one new and two known cerebrosides (ceramide derivatives), two nucleosides, and three sterols. Cordycerebroside A (1), the new cerebroside, along with soyacerebroside I (2) and glucocerebroside (3) inhibited the accumulation of pro-inflammatory iNOS protein and reduced the expression of COX-2 protein in LPS-stimulated RAW264.7 macrophages. This is the first study on the isolation of cerebrosides with anti-inflammatory activity from this TCM.

  anti-inflammatory, cerebrosides, Cordyceps militaris, LPS-stimulated RAW264.7 macrophages, fungal fruiting bodies

  NCBI PubMed ID: 26853111
  Publication DOI: 10.1021/acs.jafc.5b05931
  Journal NLM ID: 0374755
  Publisher: American Chemical Society
  Correspondence: Wu Y-C ; Chang F-R
  Institutions: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, School of Pharmacy, College of Pharmacy, China Medical University, Taichung, Taiwan, Institute of Microbiology, Chung Shan Medical University, Taichung, Taiwan, Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street, Cairo, Egypt, Department of Cosmeceutics, China Medical University, Taichung, Taiwan, Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, Taiwan, Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan, Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan, Research Center for Chinese Herbal Medicine, China Medical University, Taichung, Taiwan, Center of Molecular Medicine, China Medical University Hospital, Taichung, Taiwan
  Methods: 13C NMR, 1H NMR, NMR-2D, PCR, GC-MS, DNA techniques, TLC, ESI-MS, Western blotting, extraction, optical rotation measurement, CC, RP-HPLC, cell growth, HR-ESI-MS, determination of NO production, sonication, DEPT, immunoprecipitation assay, anti-inflammatory assays
  
   
  
  Cordyceps militaris
  CSDB ID 51035 (all data & tools)
• Article ID: 9374
  Mahmoud MM, Abdel-Razek AS, Hamed A, Soliman HSM, Ponomareva LV, Thorson JS, Shaaban KA, Shaaban M "RF-3192C and other polyketides from the marine endophytic Aspergillus niger ASSB4: structure assignment and bioactivity investigation" - Medicinal Chemistry Research 30 (2021) 647–654
  

  Chemical investigation of the methanolic extract of endophytic Aspergillus niger SB4, isolated from the marine alga Laurencia obtuse, afforded the pentacyclic polyketide, RF-3192C (1), the dimeric coumarin orlandin (2), fonsecin B (3), TMC-256A1 (4), cyclo-(Leu-Ala) (5), and cerebroside A (6).The chemical structure of RF-3192C (1) is assigned herein for the first time using 1D/2D NMR and HRESI-MS. Additionally, the revision of the NMR assignments of orlandin (2) was reported herein as well. Investigation of the antimicrobial activities of isolated compounds revealed the high activity of RF-3192C (1) against Pseudomonas aeruginosa and Bacillus subtilis, and moderate activity against yeast. Moreover, an in vitro cytotoxic activity against liver (HEPG2), cervical (HELA), lung (A549), prostate (PC3), and breast (MCF7) cancer cell lines of the isolated compounds was evaluated. The isolation and taxonomical characterization of the producing fungus was reported as well.

  polyketides, biological activities, Aspergillus niger, marine-endophyte, RF-3192C

  Publication DOI: 10.1007/s00044-020-02658-6
  Journal NLM ID: 9211347
  Publisher: Cambridge, MA: Birkhäuser Boston; New York: Springer
  Correspondence: mshaaba@gmail.com
  Institutions: Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, USA, Pharmacognosy Department, Faculty of Pharmacy, Helwan University, Helwan, Egypt, Organic and Bioorganic Chemistry, Faculty of Chemistry, Bielefeld University, Bielefeld, Germany, Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Giza, Egypt, Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City, Egypt, Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of Kentucky, Lexington, USA, Chemistry of Natural Compounds Department, Division of Pharmaceutical Industries, National Research Centre, Giza, Egypt
  Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, TLC, extraction, microscopy, CC, cell growth, HR-ESI-MS, cytotoxicity assay, DNA extraction, evaporation, antimicrobial assay, filtration, BLAST
  
   
  
  Aspergillus niger ASSB4
  CSDB ID 51042 (all data & tools)