CSDB: Search results

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Neisseria [ICD11: XN69X

]

NCBI PubMed ID: 18642671
Journal NLM ID: 8202424
Publisher: Mary Ann Liebert
Correspondence: dcstein

umd.edu
Institutions: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA

Monoclonal antibodies (MAbs) that bind neisserial lipooligosaccharides (LOS) have been widely used in structural studies of these glycolipids. MAb 2-1-L8 binds LOS with a lactosyl a chain (Gal β1-4 Glc β1-4 [Glc-NAc α1-2 Hep2 α1-3] Hep1 α1-KDO) and at least one phosphoethanolamine (PEA) substitution of Hep2, but the requirement for PEA substitution and/or the exact position of this substitution, cyclic or exocyclic, remains unclear. In order to clarify the exact specificity of this MAb, we engineered an isogenic family of lpt mutants that each make LOS with a lactosyl a chain, but that lacked cyclic (-3Hep2), exocyclic (-6Hep2), or any PEA residues. Mass spectrometry showed that mutants that lack either Lpt3 or Lpt6 make small amounts of LOS with two PEA substitutions. Thus, each enzyme is able to phosphoethanolaminylate the alternate site, albeit with low efficiency. LOS made by the mutant that lacked both Lpt3 and Lpt6 was devoid of PEA. LOS made by the ∆lpt3 mutant did not bind MAb 2-1-L8 on Western blot analysis, whereas ∆ pt6 LOS did. Analysis of intact mutants by fluorescence-activated cell sorting confirmed that PEA substitution at 3Hep2, but not at 6Hep2, is needed for optimal binding of MAb 2-1-L8. These data confirm that the MAb 2-1-L8 epitope requires a -3Hep2 cyclic PEA substitution for optimal conformation and that this MAb specifies the PEA-3Hep2 lactosyl LOS structure

conformation, monoclonal antibodies, epitopes, spectrometry, Neisseria gonorrhoeae, lipooligosaccharides, Binding Sites

Structure type: oligomer
Location inside paper: p. 72, fig 1
Aglycon: lipid A
Trivial name: core oligosaccharide L8
Compound class: core oligosaccharide, LOS, LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130659,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_140087,IEDB_140088,IEDB_140089,IEDB_140090,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_175430,IEDB_190606,IEDB_2189047,IEDB_226300,IEDB_418765,IEDB_418766,IEDB_418767,IEDB_418768,IEDB_418769,IEDB_418770,IEDB_419428,IEDB_419429,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88

Methods: PCR, SDS-PAGE, MALDI-TOF MS, serological methods, genetic methods, immunoblotting, fluorescence-activated cell sorter analysis
Biological activity: serological data
Biosynthesis and genetic data: genetic data

Related record ID(s): 4177, 5391
NCBI Taxonomy refs (TaxIDs): 482
Reference(s) to other database(s): GTC:G66058QA
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@@H](O[C@H]2[C@@H](OP(=O)(O)OCCN)[C@H](O)[C@@H]([C@@H](O)CO)O[C@@H]2O[C@@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](O[C@]4(C(=O)O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O4)C[C@](O)(C(=O)O)O[C@@H]3[C@H](O)CO)O[C@H]([C@@H](O)CO)[C@H]2O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)O[C@H](CO)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Cryptococcus neoformans ATCC H99

(previously named: Cryptococcus neoformans A H99; later renamed to: Cryptococcus neoformans var. grubii H99)
(NCBI TaxID 235443, species name lookup)

Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Associated disease: infection due to Cryptococcus neoformans [ICD11: XN3EH

]

NCBI PubMed ID: 20070311
Publication DOI: 10.1111/j.1462-5822.2010.01430.x
Publisher: New York: J. Wiley & Sons Ltd
Editors: Soldati T, Gow N, Grinstein S, Hartland E, Locker JK, Scherf A, Shao F
Correspondence: nosanchu

aecom.yu.edu
Institutions: Departments of Medicine (Division of Infectious Diseases) and Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA

The polysaccharide capsule of the fungus Cryptococcus neoformans is its main virulence factor. In this study, we determined the effects of mannitol and glucose on the capsule and exopolysaccharide production. Growth in mannitol significantly increased capsular volume compared with cultivation in glucose. However, cells grown in glucose concentrations higher than 62.5 mM produced more exopolysaccharide than cells grown in mannitol. The fibre lengths and glycosyl composition of capsular polysaccharide from yeast grown in mannitol was structurally different from that of yeast grown in glucose. Furthermore, mannitol treatment of mice infected intratracheally with C. neoformans resulted in fungal cells with significantly larger capsules and the mice had reduced fungal dissemination to the brain. Our results demonstrate the capacity of carbohydrate source and concentration to modify the expression of a major virulence factor of C. neoformans. These findings may impact the clinical management of cryptococcosis.

capsular polysaccharide, mannitol, Cryptococcus neoformans, cryptococcosis

Structure type: structural motif or average structure
Location inside paper: p.740
Compound class: CPS, glucuronoxylomannan (GXM)
Contained glycoepitopes: IEDB_114701,IEDB_115136,IEDB_115576,IEDB_130701,IEDB_140116,IEDB_140630,IEDB_144983,IEDB_145668,IEDB_152206,IEDB_164174,IEDB_167188,IEDB_174332,IEDB_423153,IEDB_76933,IEDB_983930,SB_197,SB_44,SB_67,SB_72

Methods: GC-MS, ELISA, composition analysis, light scattering, statistical analysis, zeta potential measurement, fluorescence-activated cell sorter analysis
Comments, role: GXM is formed by a linear (1-3)-a-D-mannopyranan main chain bearing b-d-xylopyranosyl in b(1-) or b(1-4) linkages, (-2)b-D-glucopyranosyluronic acid (GlcA) attached to every third mannose, on average, and 6-O-acetyl substituents

Related record ID(s): 41933, 42147
NCBI Taxonomy refs (TaxIDs): 235443
Show glycosyltransferases

There is only one chemically distinct structure:

*O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O[C@@H]2[C@H](O)[C@@H](O[C@H]3[C@H](O)[C@@H](CO)O[C@H](*)[C@H]3O[C@@H]3O[C@H](C(=O)O)[C@@H](O)[C@H](O)[C@H]3O)O[C@H](CO)[C@H]2O)[C@H]1O[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Cryptococcus neoformans ATCC H99

(previously named: Cryptococcus neoformans A H99; later renamed to: Cryptococcus neoformans var. grubii H99)
(NCBI TaxID 235443, species name lookup)

Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Associated disease: infection due to Cryptococcus neoformans [ICD11: XN3EH

aecom.yu.edu
Institutions: Departments of Medicine (Division of Infectious Diseases) and Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA

capsular polysaccharide, mannitol, Cryptococcus neoformans, cryptococcosis

Structure type: structural motif or average structure
Location inside paper: p.740
Compound class: CPS, galactoxylomannan (GalXM)
Contained glycoepitopes: IEDB_114701,IEDB_130701,IEDB_134624,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_144983,IEDB_145668,IEDB_151528,IEDB_152206,IEDB_164174,IEDB_167188,IEDB_174332,IEDB_190606,IEDB_983930,SB_163,SB_165,SB_166,SB_187,SB_195,SB_197,SB_44,SB_67,SB_7,SB_72,SB_88

Methods: GC-MS, ELISA, composition analysis, light scattering, statistical analysis, zeta potential measurement, fluorescence-activated cell sorter analysis
Comments, role: The structure is described ambiguously in the paper. Corrected using branched component of galactoxylomannan (GalXM) from [Doering TL, 2000] and [Vaishnav VV, 1998].

Related record ID(s): 41714, 42147
NCBI Taxonomy refs (TaxIDs): 235443
Reference(s) to other database(s): GTC:G16353MJ
Show glycosyltransferases

There is only one chemically distinct structure:

*OC[C@H]1O[C@H](OC[C@H]2O[C@H](*)[C@H](O)[C@@H](O)[C@H]2O)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@@H]5OC[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O[C@@H]3OC[C@@H](O)[C@H](O)[C@H]3O)[C@H](O[C@@H]3OC[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Cryptococcus neoformans ATCC H99

(previously named: Cryptococcus neoformans A H99; later renamed to: Cryptococcus neoformans var. grubii H99)
(NCBI TaxID 235443, species name lookup)

Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Associated disease: infection due to Cryptococcus neoformans [ICD11: XN3EH

aecom.yu.edu
Institutions: Departments of Medicine (Division of Infectious Diseases) and Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA

capsular polysaccharide, mannitol, Cryptococcus neoformans, cryptococcosis

Structure type: homopolymer
Location inside paper: p.740
Trivial name: galactan
Compound class: CPS, EPS, galactan, galactomannan
Contained glycoepitopes: IEDB_134624,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_163,SB_7

Methods: GC-MS, ELISA, composition analysis, light scattering, statistical analysis, zeta potential measurement, fluorescence-activated cell sorter analysis
Comments, role: backbone of galactoxylomannan (GalXM)

Related record ID(s): 41714, 41933
NCBI Taxonomy refs (TaxIDs): 235443
Reference(s) to other database(s): GTC:G83948UI, CCSD:50057, CBank-STR:4988
Show glycosyltransferases

There is only one chemically distinct structure:

*OC[C@H]1O[C@H](*)[C@H](O)[C@@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize