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Structure type: structural motif or average structure ; 30300
Compound class: polysaccharide, mannan
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_141832,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_241100,IEDB_76933,IEDB_857732,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_67,SB_72

• Article ID: 9391
  Li H, Xie W, Sun H, Cao K, Yang X "Effect of the structural characterization of the fungal polysaccharides on their immunomodulatory activity" - International Journal of Biological Macromolecules 164 (2020) 3603-3610
  

  The immunomodulatory effects of the four extracellular polysaccharides, namely WPA, WPB, AP2A, and TP1A, which were isolated from the fermented broth of Aspergillus aculeatus, A. terreus and Trichoderma sp. KK19L1, were investigated in vitro. WPA, WPB, AP2A, and TP1A were not toxic to RAW264.7 cells. These polysaccharides enhanced cell viability. WPA, WPB, AP2A, and TP1A showed increased immunomodulatory effect by strengthening the phagocytic activity and enhancing the release of NO, TNF-α and IL-6 from RAW264.7 cells. WPA, WPB, AP2A, and TP1A exhibited different immunomodulatory activity in vitro due to their different structural characterizations, and their immunoregulatory effects decreased successively in the following order: WPA, WPB, AP2A, and TP1A. The extracellular polysaccharides WPA, WPB, AP2A, and TP1A had potent immunomodulatory effects and could be used as potential immunomodulatory agents in the fields of functional food and medicine.

  extracellular polysaccharide, immunomodulatory activity, Structural characterization, fungus

  NCBI PubMed ID: 32860795
  Publication DOI: 10.1016/j.ijbiomac.2020.08.189
  Journal NLM ID: 7909578
  Publisher: Butterworth-Heinemann
  Correspondence: yangxihong63@163.com
  Institutions: College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Shandong, China, Shandong Provincial Key Laboratory of Biochemical Engineering, Shandong, China, Qingdao Academy of Agricultural Sciences, Shandong, China, College of Electromechanical Engineering, Qingdao University of Science and Technology, Shandong, China
  Methods: 13C NMR, 1H NMR, ELISA, biological assays, ion-exchange chromatography, extraction, optical rotation measurement, cell growth, determination of NO production, precipitation, SEM, Congo Red assay
  
   
  
  Aspergillus terreus W6
  CSDB ID 51108 (all data & tools)
• Article ID: 9392
  Li H, Gao T, Wang J, Tian S, Yuan X, Zhu H "Structural identification and antitumor activity of the extracellular polysaccharide from Aspergillus terreus" - Process Biochemistry 51(10) (2016) 1714-1720
  

  A water-soluble extracellular polysaccharide, AP2A, was purified from the culture medium of Aspergillus terreus. AP2A was mainly composed of mannose with the average molecular weight of about 30.3 kDa. By chemical, IR, GC/MS, 1D and 2D NMR spectroscopic analysis, the backbone of AP2A was found to be composed of [→3)-α-D-Manp(1→], [→6)-α-D-Manp(1→] and [→2,6)-α-D-Manp(1→] with [α-D-Manp-(1→2)-α-D-Manp(1→] and [α-D-Manp-(1→] as branches substituted at the C-2 position of [→2,6)-α-D-Manp(1→]. AP2A showed an anti-proliferative effect in vitro on HeLa and MCF-7 cells in a dose- and time-dependent manner. Flow cytometry showed that AP2A inhibited HeLa cell proliferation by arresting cell cycle in G2/M phase, and Annexin V-FITC/PI double staining assay indicated that AP2A could also induce HeLa cells apoptosis. These results suggest that the extracellular polysaccharide, AP2A from Aspergillus terreus, has possible chemotherapeutic applications.

  structure, extracellular polysaccharide, antitumor activity, Aspergillus terreus

  Publication DOI: 10.1016/j.procbio.2016.08.001
  Journal NLM ID: 9211419
  Publisher: Barking, Essex: Elsevier Applied Science
  Correspondence: Li H ; Zhu H
  Institutions: College of Chemistry and Environment Science, Hebei University, Baoding, China, College of Pharmaceutical Science, Hebei University, Baoding, China, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China
  Methods: 13C NMR, 1H NMR, methylation, IR, GC-MS, acid hydrolysis, biological assays, HPLC, optical rotation measurement, acetylation, reduction, CC, cell growth, flow cytometry analysis, HPGPC, precipitation, phenol-sulfuric acid assay, derivatization, Bradford method, evaporation, MTT, carbazole method, filtration, cell proliferation assay, apoptosis assay, staining
  
   
  
  Aspergillus terreus W6
  CSDB ID 51112 (all data & tools)