Investigation of the fungus Phoma herbarum PSU-H256 isolated from a leaf of Hevea brasiliensis resulted in the isolation of eight terezine derivatives (E-L) together with four known compounds. Their structures were established by analysis of spectroscopic evidence. For terezines E and H, their structures were confirmed by single-crystal X-ray diffraction crystallography. In addition, the absolute configuration at C-7 in terezine E was established by Mosher's method. Terezines K and L were tested for antibacterial, antimalarial, antimycobacterial and cytotoxic activities, but were inactive.
Phoma herbarum, Euphorbiaceae, Hevea brasiliensis, pyrazine, pyrazinone
NCBI PubMed ID: 26626161Publication DOI: 10.1016/j.phytochem.2015.11.009Journal NLM ID: 0151434Publisher: Elsevier
Correspondence: Rukachaisirikul V
Institutions: National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Klong Luang, Thailand, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand, Natural Products Research Center of Excellence and Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand, Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
Methods: 13C NMR, 1H NMR, IR, TLC, biological assays, UV, extraction, microscopy, optical rotation measurement, CC, cell growth, melting point determination, antibacterial assay, cytotoxicity assay, evaporation, ECD, antimalarial assay
Found