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1. (CSDB ID: 22803) | report error |
| a-D-Glcp-(1-3)-a-D-Glcp-(1-4)-b-D-Galp-(1-7)-+ | Subst-(1-7)-+ | ?%EtN-(1--P--7)--+ | | | {{{-a-D-Glcp-(1-6)-}}}a-D-Glcp-(1-2)-D-gro-a-D-manHepp-(1-2)-D-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/(2->6)lipid A/ Subst = O-antigen (ID 22805) | Show graphically |
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Helicobacter pylori 26695 HP0159
(Ancestor NCBI TaxID 85962,
species name lookup)
]
nrc-cnrc.gc.caThe outer core region of Helicobacter pylori lipopolysaccharide of the majority of isolates contains an α-1,6-glucan polymer synthesized by the product of the HP0159 ORF. Structural studies carried out on HP0159 lipopolysaccharide mutants by a combination of chemical methods, mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that insertional inactivation of HP0159 gene in H. pylori strains 26695 and SS1 resulted in formation of a truncated lipopolysaccharide molecule characterized by the presence of a terminal dd-heptose residue in the side-chain outer core fragment and maintaining an inner core backbone structure compared with the wild-type Lewis antigen-expressing strains. Colonization studies with HP0159 mutants of two mouse-colonizing strains, SS1 and M6, confirmed their inability to successfully colonize the murine stomach
Lipopolysaccharide, structure, Helicobacter pylori, Helicobacter, colonization
Structure type: oligomer|
2. (CSDB ID: 22804) | report error |
| a-D-Glcp-(1-3)-a-D-Glcp-(1-4)-b-D-Galp-(1-7)-+ | Subst-(1-7)-+ | ?%EtN-(1--P--7)--+ | | | {{{-a-D-Glcp-(1-6)-}}}/n=0-1/-a-D-Glcp-(1-2)-D-gro-a-D-manHepp-(1-2)-D-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/(2->6)lipid A/ Subst = O-antigen (ID 22807) | Show graphically |
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Helicobacter pylori SS1
(NCBI TaxID 102617,
species name lookup)
]nrc-cnrc.gc.caThe outer core region of Helicobacter pylori lipopolysaccharide of the majority of isolates contains an α-1,6-glucan polymer synthesized by the product of the HP0159 ORF. Structural studies carried out on HP0159 lipopolysaccharide mutants by a combination of chemical methods, mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that insertional inactivation of HP0159 gene in H. pylori strains 26695 and SS1 resulted in formation of a truncated lipopolysaccharide molecule characterized by the presence of a terminal dd-heptose residue in the side-chain outer core fragment and maintaining an inner core backbone structure compared with the wild-type Lewis antigen-expressing strains. Colonization studies with HP0159 mutants of two mouse-colonizing strains, SS1 and M6, confirmed their inability to successfully colonize the murine stomach
Lipopolysaccharide, structure, Helicobacter pylori, Helicobacter, colonization
Structure type: oligomer|
3. (CSDB ID: 124313) | report error |
| a-D-Glcp-(1--P--6)--+ | Subst-(1--P--6)--a-D-Galp-(1-6)-a-D-Galp-(1-3)-b-D-Galf-(1-3)-a-D-Manp-(1-3)-a-D-Manp-(1-4)-a-D-GlcpN-(1-6)-INO-(1--P--3)--Gro-(1-1)-ALK Subst = polysaccharide (IDs 224313, 124320, 124314) | Show graphically |
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Leishmania mexicana
(NCBI TaxID 5665,
species name lookup)
Leishmania donovani
(NCBI TaxID 5661,
species name lookup)
Leishmania major
(NCBI TaxID 5664,
species name lookup)
]Leishmania promastigotes, the stage of the parasite characteristic for the sandfly vector, express an abundant glycoconjugate, called lipophosphoglycan, at their surface. Lipophosphoglycan consists of lysoalkyl-sn-glycerophosphoinositol linked to a phosphosaccharide core conserved in all species, which is connected to PO4-6Gal β1,4Man α1 repeats with species-specific substitutions at the Gal residue; the repeats are capped by conserved and species-specific oligosaccharides. Most Leishmania species also secrete an acid phosphatase, which, in Leishmania mexicana, is a filamentous complex composed of a phosphorylated glycoprotein and non-covalently associated proteo-(high-molecular-mass)phosphoglycan. The secreted acid phosphatase complex was used as an antigen to derive a panel of monoclonal antibodies (mAbs). A total of 25 mAbs (17 novel and 8 previously described) were tested by different techniques for their specificity against lipophosphoglycan and secreted acid phosphatase from several Leishmania species. This comparison and the modification of the antigens by chemical or enzymic treatments allowed a classification of the mAbs into several groups. First, from 25 mAbs examined, 22 recognize lipophosphoglycan and the enzyme complex of L. mexicana; only three are specific for secreted acid phosphatase. Two of the latter group are also directed against carbohydrate structures, whereas the third mAb recognizes the 100-kDa polypeptide of the complex. The secreted acid-phosphatase-specific class detects antigen in the flagellar pocket of promastigotes while all anti-lipophosphoglycan mAbs bind to the cell surface. Second, all 15 anti-lipophosphoglycan mAbs investigated in detail appear to be directed against the phosphosaccharide repeats or the cap structure rather than the phosphosaccharide core. Two mAbs recognize terminal cap-structures containing Man α1,2Man residues. Four antibodies are specific for L. mexicana and are probably directed against PO4-6[Glc β1,3]Gal β1,4Man α1 repeats while six mAbs react with the unmodified repeats. Two antibodies specific for Leishmania major recognize Gal β1,3-substituted repeats unique for lipophosphoglycan from this species. Analysis by immunoblotting indicates that the high-molecular-mass proteo-phosphoglycan of L. mexicana secreted acid phosphatase carries epitopes for all anti-lipophosphoglycan mAbs suggesting the presence of capped phosphosaccharide repeats while the enzymically active glycoprotein subunit is modified by caps but probably not by repeats. In the case of Leishmania donovani secreted acid phosphatase, the enzymically active polypeptide may be directly modified by repeats. The mAbs are used to characterize changes in lipophosphoglycan structure, which occur in culture during the transition of promastigotes from the logarithmic to the stationary growth phase. Furthermore, testing the mAbs against seven species demonstrates their potential for serotyping Leishmania.
Structure type: oligomer| New query | Export IDs | Home | Help |
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