Synthetic antigens that mimic the terminal hexasaccharide epitope of the O-specific polysaccharide of Vibrio cholerae O1, serotype Ogawa, were conjugated to bovine serum albumin (BSA). Conjugates with carbohydrate-to-carrier molar ratios of 15.5&rcolon;1, 9.2&rcolon;1, and 4.6&rcolon;1 were tested for immunogenicity and efficacy in mice. The role of preimmunity to BSA and the use of adjuvant in the generation of the serologic response to the O-specific polysaccharide and protection against virulent V. cholerae was examined. Preimmunity to BSA did not affect the anti-Ogawa titers but seemed to enhance the protective capacity of antiserum. All 3 conjugates were immunogenic, but adjuvant was effective at inducing higher and earlier antibody responses. In tertiary serum samples, a correlation was found between vibriocidal activity and protection. The protective capacity of antiserum was evident in serum from mice immunized with all conjugates, but it was highest in the groups that received the conjugate with the lowest level of substitution. Further studies are required to increase understanding of the reason for differential protection
Lipopolysaccharide, antigen, oligosaccharide, hexasaccharide, chemistry, correlation, disease, role, terminal, polysaccharide, serotype, group, antibodies, antibody, epitope, antibody response, antigens, generation, level, mice, protein, response, O-specific, O-specific polysaccharide, activity, serologic, immunity, Vibrio, Vibrio cholerae, Vibrio cholerae O1, vaccine, Synthetic, immunogenic, immunogenicity, serum, bound, A-protein, conjugate, conjugates, virulent, protective, vibriocidal, antiserum, protection, adjuvant, use, bovine, bovine serum albumin, BSA, capacity, carrier, diabetes, efficacy, induction, kidney, medicinal, medicinal chemistry, protective immunity, Serum Albumin, substitution, synthetic antigen
NCBI PubMed ID: 11920320Journal NLM ID: 0413675Publisher: Oxford: Oxford University Press
Correspondence: william.wade@dartmouth.edu
Institutions: Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health,Bethesda, Maryland, Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire, Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Sakado, Japan, Unite de Biochimie Structurale, Center National de la Recherche Scientifique, Unite de Recherche Associe 2185, and Unite du Cholera et des Vibrions, Centre National de Reference des Vibrions et du Cholera, Institut Pasteur, Paris, France
Methods: biological assays, serological methods
Found