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Structure type: oligomer

• Article ID: 779
  Corsaro MM, Grant WD, Grant S, Marciano CE, Parrilli M "Structure determination of an exopolysaccharide from an alkaliphilic bacterium closely related to Bacillus spp" - European Journal of Biochemistry 264(2) (1999) 554-561
  

  An exopolysaccharide obtained from an alkaliphilic bacterium closely related to Bacillus spp. was found to contain D-galactopyranuronic acid (GalpA), 2,4-diacetamido-2,4,6-trideoxy-D-glucopyranose (QuipNAc4NAc), 2-acetamido-2-deoxy D-mannopyranuronic acid (ManpNAcA) and one uncommon unit of D-galactopyranuronic acid with the carboxyl group amide-linked to glycine [GalpA(Gly)]. The polysaccharide was studied by one-dimensional and two-dimensional 1H NMR and 13C NMR spectroscopy both on native polysaccharide and on monosaccharides and oligosaccharides obtained from methanolysis and from anhydrous HF solvolysis. The following linear structure of the repeating unit was established: →3)-α-D-GalpA(Gly)-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GalpA-(1→3)-α-D-QuipNAc4NAc-(1→. A preliminary phylogenetic assignment for the bacterium is also reported.

  structure, determination, bacteria, exopolysaccharide, glycine, Bacillus, phylogenetic analysis, Structure determination, alkaliphilic

  NCBI PubMed ID: 10491103
  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Correspondence: parrilli@unina.it
  Institutions: Dipartimento di Chimica Organica e Biologica, Universita di Napoli Federico II, Italy, Department of Microbiology and Immunology, University of Leicester, UK
  Methods: NMR-2D, FAB-MS, NMR, HF solvolysis, Smith degradation, MALDI-MS, methanolysis
  
   
  
  Bacillus sp. 15.1.1.tr
  CSDB ID 1329 (all data & tools)

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Structure type: oligomer
Contained glycoepitopes: IEDB_130648,IEDB_137340,IEDB_137473,IEDB_141501,IEDB_141807,IEDB_151531

• Article ID: 836
  Jachymek W, Petersson C, Helander A, Kenne L, Niedziela T, Lugowski C "Structural studies of the O-specific chain of Hafnia alvei strain 32 lipopolysaccharide" - Carbohydrate Research 292 (1996) 117-128
  

  The structure of the O-specific side-chain of the Hafnia alvei strain 32 lipopolysaccharide has been investigated. Methylation analysis, partial acid hydrolysis, Smith degradations, NMR spectroscopy, MALDI-TOF and FAB mass spectrometry in combination wilh collision-induced decomposition MS/MS were the principal methods used. It is concluded that the polysaccharide is composed of pentasaccharide repeating units having the following structure which is partially 0-acetylated in the 2- (20%) and 3- (50%) position of the →4)-a-D-GalpA-(l→ residue. -4)[Ac(1-3),Ac(1-2)]aDGalpA(1-2)aLRhap(1-4)bDGalp(1-3)[Ac(1-2)]bDGalpN(l-4)[Ac(1-2)]aDGlcpN(l-. A MALDI-TOF mass spectrum of the O-specific chains indicated that they consisted of up to 16 repeating units.

  Lipopolysaccharide, LPS, strain, structural, chain, O-specific, Hafnia alvei, structural studies, Hafnia, D-galacturonic acid

  NCBI PubMed ID: 8870241
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Science, Czerska 12, 53-114 Wroclaw, Poland, Department of Chemistry, Swedish University of Agricultural Sciences, P.O.Box 7015, S-750 07 Uppsala, Sweden, Swedish NMR Centre, P.O.Box 17035, S-104 06 Stockholm, Sweden
  Methods: methylation, deacetylation, NMR-2D, FAB-MS, partial acid hydrolysis, carboxyl reduction, Smith degradation, MS/MS, MALDI-TOF MS
  
   
  
  Hafnia alvei 32
  CSDB ID 2584 (all data & tools)