Exopolysaccharide (EPS-1) was isolated from Aureobasidium pullulans CGMCC 23063 and purified with DEAE 650M and Sephadex G-100 column chromatography. The structural characteristics and immunomodulatory activity of EPS-1 were investigated. The results showed EPS-1 (294.9 KDa) was β-glucan consisting of the backbone (1→3)-linked β-Glcp and β-(1,6)-branches by Ion chromatography, gas chromatography-mass spectrometry, and 1D/2D NMR spectroscopy. The SEM and AFM showed that EPS-1 composed spheres joined by triple-helix conformation into chains and circles. In vitro cell experiments, EPS-1 displayed immunological activity on the RAW264.7 cells. After being treated with EPS-1 (400 μg/mL), the concentration of NO, TNF-α, and IL-6 could reach 17.05 μM, 448.46 pg/mL, and 15.18 pg/mL, respectively. In addition, the cytokines mRNA levels also demonstrated the above results at the molecular level. The western-blot results demonstrated that the expression of phosphorylated p65 and p-erk associated with cytokine release, was increased with the dose of EPS-1.
exopolysaccharides, pathway, immunomodulatory activity, Structural characterization, pullulan, Aureobasidium pullulans
NCBI PubMed ID: 35450628Publication DOI: 10.1016/j.carbpol.2022.119366Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: C. Zhong
; S. Jia
Institutions: State Key Laboratory of Food Nutrition & Safety, Tianjin University of Science and Technology, Tianjin, PR China, Key Laboratory of Industrial Fermentation Microbiology, (Ministry of Education), Tianjin University of Science and Technology, Tianjin, PR China, Tianjin Key Laboratory of Epigenetics for Organ Development in Preterm Infants, Tianjin, PR China
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, anion-exchange chromatography, Western blotting, FTIR, UV, cytokine analysis, extraction, statistical analysis, fermentation, determination of NO production, HPGPC, SEM, Congo Red assay, AFM, immunomodulatory activity analysis, ROS measurement
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