Agrobacterium tumefaciens is a Gram-negative, phytopathogenic bacterium and is characterized by an unique mode of action on dicotyledonous plants: it is able to genetically modify the host, and because of this feature, it is used as a tool for transgenic plants. Many experiments have demonstrated that lipopolysaccharides (LPSs) play an important role for the disease development, as they are involved in the adhesion process of the bacterium on the plant cell wall. Despite the wealth of information on the role of LPS on phytopathogenesis, the present paper appears as the first report on the molecular primary structure of the O-chain produced from Agrobacterium. Its repeating unit was determined by means of chemical and spectroscopical analysis, and has the following structure: (3)-α-D-Araf-(1→3)-α-L-Fucp-(1→.
Lipopolysaccharide, lipopolysaccharides, chemistry, metabolism, microbiology, pathogenicity, strain, structural, polysaccharide, determination, structural determination, O-chain, Carbohydrate Conformation, Agrobacterium, Agrobacterium tumefaciens, Plant Diseases, Plants
NCBI PubMed ID: 12071951Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: decastro@unina.it
Institutions: Dipartimento di Chimica Organica and Biochimica, Universita di Napoli, Complesso Universitario Monte Sant' Angelo, Napoli, Italy, Dipartimento di Biologia Vegetale, Universita di Napoli, Italy
Methods: NMR-2D, NMR, sugar analysis, methanolysis
Found