Natural products that inhibit cell cycle progression may have potential as anticancer agents. In this study, cell cycle inhibition of microbial culture extracts was screened by fluorescent images using HeLa/Fucci2 cells. The culture extract of a fungus, Akanthomyces sp., inhibited the cell cycle progression at the S/G2/M phases, and bioassay-guided fractionation of the extract afforded three previously undescribed aphidicolin derivatives, namely akanthomins A-C, and an undescribed chromone glycoside, specifically 9-hydroxyeugenetin 9-O-β-d-(4-O-methyl)glucopyranoside, in addition to aphidicolin. The chemical structures of these compounds were elucidated by spectroscopic analysis and chemical derivatization. Using a flow cytometer, akanthomin A and aphidicolin were found to inhibit cell cycle progression at the S phase.
pharmaceutical, fungus, cordycipitaceae, Akanthomyces sp., Aphidicolin derivative, cell cycle inhibitor
NCBI PubMed ID: 37806468Publication DOI: 10.1016/j.phytochem.2023.113885Journal NLM ID: 0151434Publisher: Elsevier
Correspondence: Y. Hitora
; S. Tsukamoto
Institutions: Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, 862-0973, Japan
Methods: 13C NMR, 1H NMR, UV, extraction, optical rotation measurement, RP-HPLC, HR-ESI-MS, cytotoxicity assay, MPLC, cultivation, ECD, fluorescent microscopy, cell cycle analysis
Found