Armillaria luteo-virens (A. luteo-virens) is a kind of edible fungus mainly exists in Qinghai-Tibet of China, but at present only very few studies focus on the bioactivities of its polysaccharides. This study aimed to purify and characterize the structure features of a novel intracellular polysaccharide (ALP-A) derived from A. luteo-virens and explore its potential anti-tumor and immunomodulatory activities. Through systematic separation and purification, we obtained a homogeneous ALP-A with an average molecular weight of 23693Da. Structural analysis indicated that ALP-A was mainly composed of glucose and mannose with a molar ratio of 6.02:1. The repeating unit of ALP-A was →4)-α-D-Glcp-(1→ backbone with α-Glcp-(1→ and α-Manp-(6→ side chains which branched at O-2 position. The anti-tumor assays in vivo suggested that ALP-A could effectively restrain S180 solid tumor growth, protect immune organs and promote the secretion of cytokines (IL2, IL6 and TNF-α) in serum. Besides, in vitro immunomodulatory assays indicated that ALP-A could improve proliferation, phagocytic capacity and raise the level of NO and cytokines in Raw264.7 cells. These results demonstrate that ALP-A which possess potential antitumor and immunomodulatory abilities can be developed as a new functional food.
immunomodulatory ability, fungal polysaccharide, anti-tumor effect, Armillaria luteo-virens
NCBI PubMed ID: 37738818Publication DOI: 10.1016/j.carres.2023.108945Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M. Wang
Institutions: Laboratory of Molecular Oncology, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610064, China, Laboratory Animal Center, West China School of Basic Medical Science & Forensic Medicine, Sichuan University, Chengdu, 610041, China, Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China
Methods: 1H NMR, methylation, GC-MS, sugar analysis, FTIR, HPLC, cytokine analysis, extraction, statistical analysis, cell viability assay, HPGPC, antitumor activity assay, immunomodulatory activity analysis, animal experiment
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