A sulfated galactoglucan (3-SS) was discovered in Antrodia cinnamomea with antiproliferative and anti-inflammatory activities. Chemical identification of 3-SS resulted in the determination of a partial repeat unit as a 2-O sulfated 1,3-/1,4-linked galactoglucan with a two-residual 1,6-O-β-Glc branch on the 3-O position of a Glc, by monosaccharide analysis and 1D and 2D NMR spectroscopy. The anti-inflammation effects of 3-SS on RAW264.7 macrophage cells, such as IL-6 inhibition, restoration of LPS-induced IκB protein degradation, and inhibited LPS-induced TGFRII protein degradation, were confirmed to occur via AKT, ERK1/2, and p-38. In addition, 3-SS impaired the proliferation of H1975 lung cancer cells through EGFR/ERK/slug signaling. This is the first finding of 2-O sulfated 1,3-/1,4-galactoglucan with 1,6-β-Glc branches with dual functions of anti-inflammatory and antiproliferative activities.
sulfated polysaccharides, Anti-inflammatory effects, Antrodia cinnamomea, antiproliferation
NCBI PubMed ID: 37100312Publication DOI: 10.1016/j.ijbiomac.2023.124559Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: C.C. Chang
Institutions: National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan, ROC, Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan, ROC, Institute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, ROC, School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ELISA, HPAEC, immunoblotting, extraction, statistical analysis, SEC
Found