In the present study, three novel antioxidant polysaccharides (G-1, AG-1, and AG-2) were isolated and purified from Pleurotus ferulae using mouse erythrocyte hemolysis inhibitory activity as an indicator. These components showed antioxidant activity at the chemical and cellular levels. Given that G-1 displayed superior performance in protecting the human hepatocyte L02 cells against oxidative damage caused by H2O2 compared to AG-1 and AG-2 and had a higher yield and purification rate, the detailed structure of G-1 was further characterized. G-1 mainly contains six kinds of linkage type units as A: →4,6)-α-d-Glcp-(1→, B: →3)-β-d-Glcp-(1→, C: →2,6)-β-d-Glcp-(1→, d: β-d-Manp(1→, E: →6)-β-d-Galp-(1→, F: →4)-β-d-Glcp-(1→. Finally, the potential in vitro hepatoprotective mechanism of G-1 was discussed and elucidated. Results suggested that G-1 can protect L02 cells from H2O2-induced damage by reducing the leakage of AST and ALT from the cytoplasm, enhancing the activities of SOD and CAT, and suppressing lipid peroxidation and production of LDH. G-1 could further reduce the production of ROS, stabilize mitochondrial membrane potential and maintain cell morphology. Hence, G-1 could be a valuable functional food with antioxidant and hepatoprotective activities.
Structural characterization, antioxidant and hepatoprotective activity, separation and purification of Pleurotus ferulae polysaccharide (PFLP)
NCBI PubMed ID: 37290546Publication DOI: 10.1016/j.ijbiomac.2023.125124Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: F. Liu
; T.B. Ng
Institutions: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China, Department of Microbiology, The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, GC, FTIR, composition analysis, HPLC, extraction, statistical analysis, morphological analysis, antioxidant activities, cytotoxicity assay, SEM, Congo Red assay, conformational analysis, UV-vis, hepatoprotective activity assay
Found