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Structure type: cyclic polymer repeating unit ; n=1, 36070
Trivial name: a water-soluble EPS (ASP-1)
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_140629,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_158538,IEDB_190606,IEDB_2346541,IEDB_420417,IEDB_420418,IEDB_420419,IEDB_420421,IEDB_857742,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72

• Article ID: 9651
  Peng B, Liu Y, Kraithong S, Mo L, Gao Z, Huang R, Zhang X "A New Exopolysaccharide of Marine Coral-Associated Aspergillus pseudoglaucus SCAU265: Structural Characterization and Immunomodulatory Activity" - Journal of Fungi 9(11) (2023) 1057
  

  Recent studies have found that many marine microbial polysaccharides exhibit distinct immune activity. However, there is a relative scarcity of research on the immunomodulatory activity of marine fungal exopolysaccharides. A novel water-soluble fungal exopolysaccharide ASP-1 was isolated from the fermentation broths of marine coral-associated fungus Aspergillus pseudoglaucus SCAU265, and purified by Diethylaminoethyl-Sepharose-52 (DEAE-52) Fast Flow and Sephadex G-75. Structural analysis revealed that ASP-1 had an average molecular weight of 36.07 kDa and was mainly composed of (1→4)-linked α-D-glucopyranosyl residues, along with highly branched heteropolysaccharide regions containing 1,4,6-glucopyranosyl, 1,3,4-glucopyranosyl, 1,4,6-galactopyranosyl, T(terminal)-glucopyranosyl, T-mannopyranosyl, and T-galactopyranosyl residues. ASP-1 demonstrated significant effects on the proliferation, nitric oxide levels, and the secretion of cytokines TNF-α and IL-6 in macrophage RAW264.7 cells. Metabolomic analysis provided insights into the potential mechanisms of the immune regulation of ASP-1, suggesting its involvement in regulating immune function by modulating amino acid anabolism, particularly arginine synthesis and metabolism. These findings provide fundamental scientific data for further research on its accurate molecular mechanism of immunomodulatory activity.

  exopolysaccharide, immunomodulatory activity, amino acid metabolism, coral-associated Aspergillus pseudoglaucus SCAU265

  NCBI PubMed ID: 37998863
  Publication DOI: 10.3390/jof9111057
  Journal NLM ID: 101671827
  Publisher: Basel, Switzerland: MDPI AG
  Correspondence: R. Huang
  Institutions: Institute for Environmental and Climate Research, Jinan University, Guangzhou 511443, China, University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China, Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China
  Methods: 13C NMR, NMR-2D, GC-MS, sugar analysis, FTIR, extraction, statistical analysis, methylation (Hakomori method), fermentation, H NMR, HPGPC, ethanol precipitation, UV-vis, metabolomics analysis, immunomodulatory activity
  
   
  
  Aspergillus pseudoglaucus SCAU265
  CSDB ID 41074 (all data & tools)