Chemical exploration of solid-state cultures of the polypore Fomitopsis carnea afforded two new C31 lanostane-type triterpenoid glycosides, forpiniosides B (1) and C (2) together with two known derivatives, namely 3-epipachymic acid (3) and (3α,25S)-3-O-malonyl-23-oxolanost-8,24(31)-dien-26-oic acid (4). The structures of the isolated compounds were established based on HRESIMS and extensive 1D and 2D NMR experiments. All the isolated compounds were assessed for their antimicrobial and cytotoxic activities. Among the tested compounds, forpinioside B (1) exhibited significant antimicrobial activity against Staphylococcus aureus and Bacillus subtilis at MIC values comparable to gentamycin and oxytetracycline (positive controls), respectively.
antimicrobial activity, Fomitopsis carnea, lanostane glycosides, Polyporales
NCBI PubMed ID: 37560136Publication DOI: 10.3762/bjoc.19.84Journal NLM ID: 101250746Publisher: Beilstein-Institut
Correspondence: M. Stadler
Institutions: Department of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstra?e 7, 38124 Braunschweig, Germany, Institute of Microbiology, Technische Universitat Braunschweig, Spielmannstra?e 7, 38106 Braunschweig, Germany, Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt, Center of Excellence in Fungal Research, School of Science, Mae Fah Luang University, 333 Muang, Chiang Rai, 57100 Thailand, Mycotheque de l' Universite Catholique de Louvain (BCCM/MUCL), Place Croix du Sud 3, B-1348 Louvain-la-Neuve, Belgium, Department of Chemistry, Faculty of Science, University of Dschang, P. O. Box 67, Dschang, Cameroon, Department of Chemistry, Egerton University, P.O. Box 536, 20115, Njoro, Kenya
Methods: 13C NMR, 1H NMR, NMR-2D, HPLC, optical rotation measurement, fermentation, HR-ESI-MS, cytotoxicity assay, antimicrobial assay, UV-vis
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