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1. (Article ID: 2367)
 
Dmitriev BA, Kocharova NA, Knirel YA, Shashkov AS, Kochetkov NK, Stanislavsky ES, Mashilova GM
Somatic antigens of Pseudomonas aeruginosa. The structure of the polysaccharide chain of Ps. aeruginosa O:6 (Lanyi) lipopolysaccharide
European Journal of Biochemistry 125 (1982) 229-237
 

An O-antigenic lipopolysaccharide, manifesting a high serological activity in the reaction of passive haemagglutination and its inhibition, was isolated from Pseudomonas aeruginosa O:6 cells (Lanyi). Splitting-off of the lipid component by mild acid hydrolysis resulted in the polysaccharide deprived of O-specific activity. The polysaccharide chain of the lipopolysaccharide was made up exclusively of the residues of amino sugars, among which have been identified: 2-acetamido-2-deoxy-D-galactose (GalNAc), 2-acetamido-2,6-dideoxy-D-glucose (QuiNAc), 2-acetamido-2,6-dideoxy-D-galactose (FucNAc) as well as 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid [Glc(NAc)2A] found naturally for the first time. The principal methods of establishing the polysaccharide structure were 13C nuclear magnetic resonance, methylation analysis and solvolysis with hydrogen fluoride. Depending on solvolysis conditions, a disaccharide or a trisaccharide containing the diacetamidouronic acid residue was formed. From the results obtained it followed that the polysaccharide chain of the O-antigenic Ps. aeruginosa O:6 lipopolysaccharide represents an acidic hexasaminoglycan constructed of the repeating tetrasaccharide units of the following structure: → 4)DGalNAc(α1→4)DGlc(NAc)2A(β1→3)DFucNAc(α1→3)DQuiNAc(α1→.

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