Konishi T, Taguchi F, Iwaki M, Ohnishi-Kameyama M, Yamamoto M, Maeda I, Nishida Y, Ichinose Y, Yoshida M, Ishii T Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin Carbohydrate Research344(16) (2009)
2250-2254
The structure was elucidated in this paper NCBI PubMed ID:19766989 Publication DOI:10.1016/j.carres.2009.07.004 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: T. Ishii <tishiiffpri.affrc.go.jb> Institutions: Forestry and Forest Products Research Institute, 1, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-L-Rhap-(1→2)-α-L-Rhap-(1→2)-α-L-Rha-(1→, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-L-Rhap-(1→2)-α-L-Rha-(1→, which was identified in a previous study.
Konishi T, Taguchi F, Iwaki M, Ohnishi-Kameyama M, Yamamoto M, Maeda I, Nishida Y, Ichinose Y, Yoshida M, Ishii T Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin Carbohydrate Research344(16) (2009)
2250-2254
The structure was elucidated in this paper NCBI PubMed ID:19766989 Publication DOI:10.1016/j.carres.2009.07.004 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: T. Ishii <tishiiffpri.affrc.go.jb> Institutions: Forestry and Forest Products Research Institute, 1, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-L-Rhap-(1→2)-α-L-Rhap-(1→2)-α-L-Rha-(1→, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-L-Rhap-(1→2)-α-L-Rha-(1→, which was identified in a previous study.
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(later renamed to: Pseudomonas amygdali pv. tabaci 6605)
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