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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Bos taurus
Associated disease: pneumonia [ICD11: CA40

]; thrombotic meningoencephalitis [ICD11: 1D01.Y

]; septicemia [ICD11: MA15.Y

]; myocarditis [ICD11: BC42

]; inflammatory arthropathies [ICD11: FA2Z

]

The structure was elucidated in this paper
NCBI PubMed ID: 11101573
Journal NLM ID: 7505564
Publisher: American Society for Microbiology
Institutions: Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada, Departments of Pediatrics and Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 191043

The lipooligosaccharide (LOS) of Haemophilus somnus undergoes antigenic phase variation, which may facilitate evasion from the bovine host immune response and/or colonization and dissemination. However, LOS antigenic diversity in H. somnus has not been adequately investigated. In this study, monoclonal antibodies (MAbs) specific to various LOS epitopes were used to investigate antigenic variation and stability in LOS from H. somnus strains and phase variants. Clinical isolates of H. somnus exhibited intrastrain, as well as interstrain, antigenic heterogeneity in LOS when probed with MAbs to outer core oligosaccharide epitopes in an enzyme-linked immunosorbent assay (ELISA). However, epitopes reactive with MAbs directed predominately to the inner core heptose region were highly conserved. At least one epitope, which was expressed in few strains, was identified. One LOS component affected by phase variation was identified as phosphorylcholine (PCho), which is linked to the primary glucose residue. Inhibition ELISA, immunoblotting, and electrospray-mass spectrometry were used to confirm that MAb 5F5.9 recognized PCho. LOS reactivity with MAb 5F5.9 was associated with loss of most of the outer core oligosaccharide, indicating that reactivity with PCho was affected by phase variation of the glycose residues in this region. Our results indicate that outer core epitopes of H. somnus LOS exhibit a high degree of random, phase-variable antigenic heterogeneity and that such heterogeneity must be considered in the design of vaccines and diagnostic tests.

Haemophilus, Lipooligosaccharide, antigenic, epitope, phosphorylcholine, Haemophilus somnus, diversity

Structure type: oligomer
Location inside paper: Fig. 3
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_117715,IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130659,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_1391962,IEDB_1391966,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_141807,IEDB_142076,IEDB_142078,IEDB_142351,IEDB_142487,IEDB_142488,IEDB_143794,IEDB_146664,IEDB_150899,IEDB_151531,IEDB_157001,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_983931,SB_137,SB_145,SB_156,SB_165,SB_166,SB_173,SB_174,SB_187,SB_192,SB_195,SB_29,SB_6,SB_7,SB_88

Methods: SDS-PAGE, ELISA, ESI-MS, MS, de-O-acetylation, immunoblotting, statistical analysis, inhibited ELISA
Biological activity: serological data, reactivity of MAbs with H. somnus
Comments, role: core, reclassified to Histophilus somnus

NCBI Taxonomy refs (TaxIDs): 731
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](COP(=O)(O)OCC[N+](C)(C)C)O[C@@H](O[C@@H]4[C@@H]([C@@H](O)CO)O[C@H](O[C@@H]5[C@H](O[C@]6(C(=O)O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O6)C[C@](O)(C(=O)O)O[C@@H]5[C@H](O)CO)[C@@H](O)[C@H]4O[C@H]4O[C@H]([C@H](CO)OP(=O)(O)OCCN)[C@@H](O)[C@H](O)[C@@H]4O)[C@H](O)[C@H]3O)[C@@H]2O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize