A new furostanol glycoside (1) was isolated from garlic, bulbs of Allium sativum L. (Liliaceae), and the structure was established to be 26-O-β-glucopyranosyl 22-hydroxy-25(R)-5α-furostane-3β,6β,26-triol 3-O-β-glucopyranosyl(1→2)-[β-glucopyranosyl(1→3)]-β-glucopyranosyl-(1→4)-β-galactopyranoside, i.e., proto-eruboside-B. Eruboside-B (3) obtained by enzymatic hydrolysis of 1 inhibited the growth of Candida albicans in vitro (25 μg/ml MIC).
antifungal activity, Liliaceae, furostanol glycoside, Allium sativum, garlic, proto-eruboside-B, eruboside-B
Journal NLM ID: 0377775WWW link: http://ci.nii.ac.jp/naid/110003654859Publisher: Pharmaceutical Society Of Japan
Institutions: Central Research Laboratories, Wakunaga Pharmaceutical Co., Ltd., Osaka, Japan
Methods: 13C NMR, 1H NMR, gel filtration, IR, partial acid hydrolysis, TLC, acid hydrolysis, MS, enzymatic digestion, HPTLC, permethylation analysis, bioassay