The specific capsular antigen of Streptococcus pneumoniae serotype 35B was shown by a combination of 2D NMR methods and mass spectrometric and classical carbohydarate chemical techniques to be a high molecular weihgt polymer containing D-galactose, D-glucose, 2-acetamido-2deoxy-D-galactose, and ribitol (2:1:1:1). The polysaccharide repeating unit is polymerized through phosphate diester linkages to give the structure {structure}. Seventy persent of the bDGalf residues glycosidically linked to the ribitol units carry an O-acetyl sustituent.
antigen, structure, capsular, characterization, K-antigen, polysaccharide, serotype, Streptococcus, Streptococcus pneumoniae
Publication DOI: 10.1139/v95-006Journal NLM ID: 0372705Publisher: National Research Council of Canada Canada
Institutions: Institute for Biological Science, National research Council of Canada, Ottava, ON K1A 0R6, Canada, Merck Research Laboratories, West Point, PA, USA
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, GLC-EI-MS, FAB-MS
Found