3-Deoxy-d-lyxo-heptulosaric acid (DHA) has been identified as a component of plant cell walls. This sugar was found in the pectic polysaccharide rhmanogalacturonan-II as a component of the disaccharide β-l-Araf-(1→5)-d-DHAp. The structural formula and linkage position of DHA were determined by mass spectrometry of several derivatives. The lyxo configuration was determined by 1H-n.m.r. spectroscopy of a derivative of the isolated disaccharide. The d configuration was determined by partial degradation of DHA to l-glyceric acid. DHA was found to be present in the primary cell walls of many higher plants, including several dicots, two monocots, and a gymnosperm, but was not detected in two bacterial lipopolysaccharides.
Publication DOI: 10.1016/0008-6215(88)84124-7Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Complex Carbohydrate Research Center and Department of Biochemistry, University of Georgia, Russell Research Center of the United States Department of Agriculture, Athens, Georgia, U.S.A.
Methods: 1H NMR, GC-MS
Found