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1. (Article ID: 2560)
Ridout MJ, Brownsey GJ, Morris VJ, Cairns P
Physicochemical characterization of an acetan variant secreted by Acetobacter xylinum strain CR1/4
International Journal of Biological Macromolecules 16 (1994)
324-330
Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
The publication contains the following compound(s):
- Compound ID: 5822
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b-D-GlcpA-(1-2)-a-D-Manp-(1-3)-+
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-4)-b-D-Glcp-(1-4)-b-D-Glcp-(1- |
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Structure type: polymer chemical repeating unit
Trivial name: acetan variant
Compound class: EPS
Reference(s) to other database(s): GTC:G11877YH, GlycomeDB:
16444
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2. (Article ID: 6303)
Rath T, Ruhmann B, Sieber V
Systematic optimization of exopolysaccharide production by Gluconacetobacter sp. and use of (crude) glycerol as carbon source
Carbohydrate Polymers 276 (2022)
118769
The usage of polysaccharides as biodegradable polymers is of growing interest in the context of a sustainable and ecofriendly economy. For this, the production of exopolysaccharides (EPS) by Gluconacetobacter sp. was investigated. Glycerol as carbon source revealed to be beneficial compared to glucose. In addition, pure glycerol could be substituted by a crude glycerol waste stream from biodiesel production. Systematic analysis of the peptone and phosphate concentrations in glycerol-based media indicated a strong effect of peptone. Optimized parameters resulted in a titer of 25.4 ± 2.4 g/L EPS with a productivity of 0.46 ± 0.04 g*(L*h)-1. With decreasing peptone, a variation in the monomer ratios was observed. An accompanying change in molecular size distribution indicated the production of two different polysaccharides. Intensified analysis revealed the main polysaccharide to be composed of glucose (Glc), galactose (Gal), mannose (Man) and glucuronic acid (GlcA), and the minor polysaccharide of Gal, Man, ribose (Rib).
exopolysaccharide, optimization
NCBI PubMed ID: 34823788Publication DOI: 10.1016/j.carbpol.2021.118769Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: V. Sieber
tum.de>
Institutions: Chair of Chemistry of Biogenic Resources, Technical University of Munich, Campus for Biotechnology and Sustainability, 94315 Straubing, Germany, Fraunhofer IGB, Branch BioCat, 94315 Straubing, Germany, Catalysis Research Center, Technical University of Munich, 85748 Garching, Germany, The University of Queensland, School of Chemistry and Molecular Biosciences, 68 Cooper Road, St. Lucia 4072, Australia
Methods: sugar analysis, GPC, fermentation, media composition, preculture preparation, UHPLC-UV-ESI-MS/MS, crude glycerol analysis
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