Two new and three known steroidal saponins were isolated from the fresh bulbs of Lilium regale and two known saponins from those of L. henryi. The structures of the new saponins were established by extensive spectral data, hydrolysis and chemical correlation as (25R)-27-O-[(S)-3-hydroxy-3-methylglutaroyl]-spirost-5-ene-3β,27-diol 3-O-α-l-rhamnopyranosyl-(1→2)-O-[β-d-glucopyranosyl-(1→3)]-β-d-glucopyranoside and (25S)-spirost-5-ene-3β,17α,27-triol-3-O-α-l-rhamnopyranosyl-(1→2)-O-[β-d-glucopyranosyl-(1→2)-O-β-d-glucopyranosyl-(1→4)]-β-d-glucopyranoside. Inhibitory activity of the saponins on cyclic AMP phosphodiesterase was evaluated.
steroidal saponins, Liliaceae, bulbs, cyclic AMP phosphodiesterase, inhibitory activity, Lilium regale, L. Henryi, 3-hydroxy-3-methylglutaric acid
NCBI PubMed ID: 7764061Publication DOI: 10.1016/0031-9422(93)85472-4Journal NLM ID: 0151434Publisher: Elsevier
Institutions: Tokyo College of Pharmacy, Japan, School of Pharmaceutical Sciences, Toho University, Miyama, Funabashi, Chiba, Japan
Methods: 13C NMR, 1H NMR, gel filtration, partial acid hydrolysis, TLC, acid hydrolysis, HPLC, enzymatic assay, SI-MS, alkaline methanolysis
Found