Suspension-cultured cells of rice secrete α-amylase into the culture medium. It has been shown that the mature form of the α-amylase contains xylose-bearing N-linked oligosaccharide: (formula; see text) We demonstrate that suspension-cultured cells of rice secrete α-amylase containing oligomannose-type oligosaccharides in the presence of 1-deoxymannojirimycin or tris(hydroxymethyl)aminomethane. On the other hand, α-amylase purified from germinated rice seedlings contains several kinds of oligomannose-type and N-acetyllactosamine-type oligosaccharides. The processing pathway of oligosaccharide moieties in rice cells is discussed on the basis of a comparison of these oligosaccharides structures.
NCBI PubMed ID: 2143471Publication DOI: 10.1111/j.1432-1033.1990.tb19122.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Japan, Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University, Japan, Nagoya City University, College of Nursing, Nagoya, Japan, Faculty of Pharmaceutical Science, University of Tokyo, Japan
Methods: 1H NMR, SDS-PAGE, HPLC, enzymatic digestion, pulse-labeling assay
Found