Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
NCBI PubMed ID: 21215391Publication DOI: 10.1016/j.carres.2010.12.005Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden
The structure of the O-antigen polysaccharide (PS) from Escherichia coli O175 has been elucidated. Component analysis together with (1)H and (13)C NMR spectroscopy experiments were used to determine the structure. Inter-residue correlations were determined by (1)H,(1)H-NOESY, and (1)H,(13)C-heteronuclear multiple-bond correlation experiments. The PS is composed of pentasaccharide repeating units with the following structure: →2)-α-D-Glcp-(1→4)-α-D-GlcpA-(1→3)-α-D-Manp-(1→2)-α-D-Manp-(1→3)-β-D-GalpNAc-(1→. Cross-peaks of low intensity from an α-linked glucopyranosyl residue were present in the (1)H,(1)H-TOCSY NMR spectra. The α-D-Glcp residue is suggested to originate from the terminal part of the polysaccharide and consequently the biological repeating unit has a 3-substituted N-acetyl-D-galactosamine residue at its reducing end. The repeating unit of the E. coli O175 O-antigen is similar to those from E. coli O22 and O83, both of which carry an α-D-Glcp-(1→4)-D-GlcpA structural element, thereby explaining the reported cross-reactivities between the strains.
Lipopolysaccharide, NMR, Escherichia coli, biological repeating unit
Structure type: polymer chemical repeating unit
Location inside paper: p.452
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_144990,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_167071,IEDB_190606,IEDB_423153,IEDB_983931,SB_192,SB_21,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, GLC, NMR-1D
Related record ID(s): 26358, 26494
NCBI Taxonomy refs (TaxIDs): 2184074Reference(s) to other database(s): GTC:G66187AK, GlycomeDB:
6570
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There is only one chemically distinct structure:
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