Two monoterpene glycosides, tentatively named OJV-I (1) and OJV-II (2), and eight steroidal glycosides, tentatively named OJV-III (3), OJV-IV (4), OJV-V (5), OJV-VI (6), OJV-VII (7), OJV-VIII (8), OJV-IX (9) and OJV-X (10), were isolated from the butanol-soluble fraction of the fresh subterranean part of Ophiopogon japonicus KER-GAWLER cv. Nanus. Among these compounds, 1,2,3,4,5,6 and 7 were identified as l-bornelo O-β-D-glucopyranoside, l-borneol O-β-D-apiofuranosyl(1→6)-β-D-glucopyranoside, ophiopogonin B, glycoside C, ophiopogonin D, Ls-10,and ruscogenin 1-O-sulfate, respectively. The structures of compounds 8,9,and 10 were established to be (23S, 24S, 25S)-23,24-dihydroxyruscogenin l-O-[α-L-rhamnopyranosyl(1→2)] [β-D-xylopyranosyl(1→3)]-α-L-arabinopyranoside 24-O-β-D-fucopyranoside, (23S, 24S, 25S)-23,24-dihyrdroxyruscogenin 1-O-[α-L-2,3,4-tri-O-acetylrhamnopyranosyl(1→2)][β-D-xylopyranosyl(1→3)]-α-L-arabinopyranoside 24-O-β-D-fucopyranoside, and (23S, 24S, 25S)-23,24-dihydroxyruscogenin 1-O-[α-L-4-O-acetylrhamnopyranosyl(1→2)] [β-D-xylopyranosyl(1→3)]-α-L-arabinopyranoside 24-O-β-D-fucopyranoside, respectively.
steroidal glycoside, Liliaceae, ophiopogonis tuber, monoterpene glycoside, Ophiopogon japonicus cv. Nanus, (23S, 24S, 25S)-23, 24-dihydroxyruscogenin
NCBI PubMed ID: 8477511Publication DOI: 10.1248/cpb.41.566Journal NLM ID: 0377775Publisher: Pharmaceutical Society Of Japan
Institutions: School of Pharmaceutical Sciences, Showa University, Japan
Methods: 13C NMR, 1H NMR, IR, partial acid hydrolysis, acid hydrolysis, GLC, HPLC, solvolysis, CC, acetylation analysis
Found