Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 31004646Publication DOI: 10.1016/j.ijbiomac.2019.04.130Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: S. Azabou <azabousamia
yahoo.fr>
Institutions: Université de Sfax, ENIS, Laboratoire Analyse, Valorisation et Sécurité des Aliments, Sfax 3038, Tunisia., Department of Chemical Sciences, University of Naples ''Federico II'', Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy, Tunisian Olive Institute, University of Sfax, Tunisia, LLaboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia
The probiotic features of strain GM newly isolated from Tunisian spontaneously fermented goat milk and identified as Bacillus tequilensis-GM were assessed. Strain GM showed high resistance to saliva (90.64%), gastric juice (88.55%), intestinal juice (72.83%) and resistance to bile salts (65.22%), was able to act against Listeria monocytogenes ATCC 15313, Escherichia coli ATCC 25922 and Enterococcus feacalis ATCC 25912, showed high surface hydrophobicity (77.3%) and was sensitive to most of the studied antibiotics. Strain GM did not exhibit any hemolytic activity whereas it was able to produce protease, amylase and β-galactosidase. Moreover, results showed that strain GM produced high molecular weight β-(2→6)-levan with high ability to inhibit and to disrupt pathogenic biofilms and with high ability to reduce syneresis of sucrose-supplemented skimmed milk. B. tequilensis-GM can therefore be suitable to be used as starter culture in fermented dairy products, since it possesses desirable probiotic properties in addition to its ability to produce levan.
Structural characterization, levan, antibiofilm activity, Bacillus tequilensis-GM, food application, probiotic potential
Structure type: fragment of a bigger structure ; 2500000
Location inside paper: p.791, table 3, fig.2B
Trivial name: levan
Compound class: EPS
Contained glycoepitopes: IEDB_923066
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, DNA sequencing, GC-MS, sugar analysis, HPLC, SEC, assessment of probiotic, antibiofilm activity
NCBI Taxonomy refs (TaxIDs): 227866Reference(s) to other database(s): GTC:G85190RA
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,1 bDFruf 62.2 105.3 78.8 76.0 82.8 63.9
6 bDFruf 61.6 106.0 78.0 76.9 82.0 65.1
bDFruf 61.6 106.0 78.0 76.9 82.0 65.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,1 bDFruf 3.67-3.75 - 4.19 4.11 3.87 3.73-3.85
6 bDFruf 3.67-3.77 - 4.19 4.10 3.95 3.55-3.90
bDFruf 3.67-3.77 - 4.19 4.10 3.95 3.55-3.90
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,1 bDFruf 62.2/3.67-3.75 78.8/4.19 76.0/4.11 82.8/3.87 63.9/3.73-3.85
6 bDFruf 61.6/3.67-3.77 78.0/4.19 76.9/4.10 82.0/3.95 65.1/3.55-3.90
bDFruf 61.6/3.67-3.77 78.0/4.19 76.9/4.10 82.0/3.95 65.1/3.55-3.90
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,1 | bDFruf | 3.67
3.75 |
| 4.19 | 4.11 | 3.87 | 3.73
3.85 |
| 6 | bDFruf | 3.67
3.77 |
| 4.19 | 4.10 | 3.95 | 3.55
3.90 |
| | bDFruf | 3.67
3.77 |
| 4.19 | 4.10 | 3.95 | 3.55
3.90 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,1 | bDFruf | 62.2 | 105.3 | 78.8 | 76.0 | 82.8 | 63.9 |
| 6 | bDFruf | 61.6 | 106.0 | 78.0 | 76.9 | 82.0 | 65.1 |
| | bDFruf | 61.6 | 106.0 | 78.0 | 76.9 | 82.0 | 65.1 |
|
There is only one chemically distinct structure:
Found 
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