Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Salmonella enterica [ICD11:
XN5VC 
];
infection due to Escherichia coli [ICD11:
XN6P4 ]
The structure was elucidated in this paperNCBI PubMed ID: 21205000Publication DOI: 10.1111/j.1574-695X.2010.00771.xJournal NLM ID: 9315554Publisher: Elsevier
Correspondence: liubin1981
nankai.edu.cn
Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
O-antigen is a part of the lipopolysaccharide present in the outer membrane of Gram-negative bacteria, which is essential for the full function of the microorganisms and is related to bacterial virulence. Salmonella enterica and Escherichia coli are taxonomically closely related species. In this study, the O-antigen structures of S. enterica O16, O38 and E. coli O11 were determined. It was found that S. enterica O38 and E. coli O21 share the O-antigen structure, whereas those of S. enterica O16 and E. coli O11 are closely related differing only in the presence of a lateral glucose residue and O-acetylation of a mannose residue in the former. The O-antigen gene clusters of S. enterica O16, O38 and E. coli O11 were sequenced and analyzed together with that of E. coli O21 retrieved from Genbank. For each S. enterica/E. coli pair, they were found to contain the same set of genes organized in the same manner and sharing 56% to 78% overall DNA identity. These data suggest that the O-antigen gene clusters of each pair studied originated from a common ancestor, as reported for a number of other known E. coli/S. enterica pairs having identical or closely related O-antigen structures. It has become thus evident that in past the degree of relatedness between the O-antigens of S. enterica and E. coli was underestimated.
Escherichia coli, Salmonella enterica, O-antigen gene cluster, Glycomics, O-antigen diversity
Structure type: suggested polymer biological repeating unit
Location inside paper: p.263, fig.2
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_221845,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_6,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, PCR, DNA sequencing, sugar analysis, acid hydrolysis, Smith degradation, de-O-acetylation, NMR-1D
Biosynthesis and genetic data: genetic data
Related record ID(s): 4937, 10389, 11682, 20649, 23242, 26271, 26884, 26885, 26886, 26887, 26888, 26889, 27435, 28337, 30360, 108742, 115996
NCBI Taxonomy refs (TaxIDs): 28901,
2162911Reference(s) to other database(s): GTC:G92807QP, GlycomeDB:
3591
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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