We report esssentially complete 1H NMR assignments for the capsular polysaccharides from Neisseria meningitidis serotypes A, C, W-135, and Y. These polysaccharides are components of current polysaccharide vaccines against meningococcal infection and of the polysaccharide-protein conjugate vaccines under development. From these NMR data the pattern of O-acetylation was determined. O-Acetylation of the W-135 polysaccharide is reporded for the first time. We also show that, for the Types C and W-135 polysaccharides, a migration of O-acetyl groups occurs during storage in solution, and demonstrate that high field 1H NMR represents a simple and sensititve method to define the O-acetylation pattern of individual batches of these polysaccharides.
NMR, structure, Neisseria meningitidis, Neisseria, capsular, polysaccharide, capsular polysaccharide, capsular polysaccharides, polysaccharides, O-acetylation, production, vaccine, assignment, 1H NMR, pattern
Publication DOI: 10.1016/S0008-6215(96)00253-4Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: cjones@nibsc.ac.uk
Institutions: Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, UK
Methods: NMR-2D, NMR
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