Further studies by means of preparative HPLC led to the isolation of two new furostanol saponins, macrostemonoside J (1) and L (3), along with an artifact, macrostemonoside K (2) from the bulbs of Allium macrostemon. On the basis of chemical evidence and spectral analysis (^1H, ^<13>C-NMR and FAB-MS), the structure of 1 was elucidated to be 26-O-β-D-glucopyranosyl 2β,3β,22,26-tetrahydroxy-25(R)-5β-furostan 3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside. 3 was deduced to be 26-O-β-D-glucopyranosyl 2β,3β,26-trihydroxy-25(R)-5β-furostan-20(22)-ene 3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside.
furostanol saponin, Allium macrostemon, macrostemonoside J, macrostemonoside L
NCBI PubMed ID: 7805140Publication DOI: 10.1248/cpb.42.2180Journal NLM ID: 0377775Publisher: Pharmaceutical Society Of Japan
Institutions: Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, China, Department of Phytochemistry and Pharmacognosy, Meiji College of Pharmacy, Japan
Methods: 13C NMR, 1H NMR, EI-MS, FAB-MS, GC-MS, acid hydrolysis, HPLC, enzymatic digestion, FD-MS
Found