Evaluation of the constitutive polyphenolics of the whole plant extract of Epilobium hirsutum was carried out by reverse-phase HPLC. The unique ellagitannins, 2-O-galloyl 3-O-valoneoyl dilactone-(α/β)-C-4(1)-glucopyranose, 1'-monodecarboxyvaloneic acid dilactone and valoneic dilactone dioxine were isolated and characterized. The known polyphenolics, gallic, protocatechuic, ellagic, valoneic dilactone and p-coumaric acids, methyl gallate, p-methoxy gallic acid methyl ester, 6-O-galloylglucose, 1,6-di-O-galloylglucose, 2,3-di-O-galloylglucose, 1,2,6-tri-O-galloylglucose and the 3-O-glucuronides, 3-O-arabinosides and 3-O-rhamnosides of kaempferol, quercetin and myricetin, their free aglycones 3-O-galactoside and quercetin and myricetin were also identified. The structures were established by conventional methods of analysis and confirmed by H-1, C-13 NMR and negative ESI-mass spectrometry. 2D-long range selective proton decoupling and chemical shift correlation NMR experiments were applied for the new polyphenolics. The HPLC phenolic profile was used for the quality.
NMR, flavonoids, Onagraceae, Epilobium hirsutum, ellagitannins, gallotannins, 2-O-galloyl 3-O-valoneoyl dilactone-(a/b)-4C1-glucopyranose, 1’-monodecarboxyvaloneic acid dilactone, valoneic dilactone dioxine, HPLC quality assessment of the extract
Publication DOI: 10.1016/S0031-9422(97)00370-1Journal NLM ID: 0151434Publisher: Elsevier
Institutions: National Research Centre, Dokki, Cairo, Egypt, Institut für Pharmazeutische Biologie Heinrich-Heine-Universität Düsseldorf, Universitätstrasse I, 40225 Düsseldorf, Germany
Methods: 13C NMR, 1H NMR, ESI-MS, paper chromatography, RP-HPLC
Found