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Kogan G, Brisson J, Kasper DL, Von Hunolstein C, Orefici G, Jennings HJ
Structural elucidation of the novel type VII group B Streptococcus capsular polysaccharide by high resolution NMR spectroscopy
Carbohydrate Research 277(1) (1995)
1-9
a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)-+
|
-4)-a-D-Glcp-(1-4)-b-D-Galp-(1-4)-b-D-Glcp-(1- |
Show graphically |
Streptococcus sp. B VII
(Ancestor NCBI TaxID 1319,
species name lookup)
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus [ICD11:
XN3NM 
]
The structure was elucidated in this paperNCBI PubMed ID: 8548782Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Laboratory of Bacteriology and Medical Mycology, Instituto Supeiore di Sanita, Rome, Italy
The type VII capsular polysaccharide isolated from the newly discovered group B Streptococcus (GBS) strain contains D-glucose, D-galactose, N-acetyl-D-glucosamine and N-acetylneuraminic acid in the molar ratio 2:2:1:1. High-resolution one- and two-dimensional (1D and 2D) 1H and 13C NMR spectroscopy of the native and desialylated polysaccharides showed the type VII GBS capsular polysaccharide to contain the following branched hexasaccharide repeating unit: [formula: see text] Despite extensive structural similarity with the previously described GBS polysaccharides, the type VII polysaccharide showed no cross-reaction with the heterologous antisera.
group B Streptococcus, NMR spectroscopy, Type VII capsular polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: page 7
Compound class: CPS
Contained glycoepitopes: IEDB_130646,IEDB_135813,IEDB_136044,IEDB_136794,IEDB_137340,IEDB_137472,IEDB_140108,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144998,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_190606,IEDB_423120,IEDB_983931,SB_115,SB_116,SB_131,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_30,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
Methods: NMR, desialylation
Biological activity: serological data
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 3072
NCBI Taxonomy refs (TaxIDs): 1319Reference(s) to other database(s): GTC:G16684MV, GlycomeDB:
27673
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There is only one chemically distinct structure:
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Kogan G, Brisson J, Kasper DL, Von Hunolstein C, Orefici G, Jennings HJ
Structural elucidation of the novel type VII group B Streptococcus capsular polysaccharide by high resolution NMR spectroscopy
Carbohydrate Research 277(1) (1995)
1-9
b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)-+
|
-4)-b-D-Glcp-(1-4)-a-D-Glcp-(1-4)-b-D-Galp-(1- |
Show graphically |
Streptococcus sp. B VII
(Ancestor NCBI TaxID 1319,
species name lookup)
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus [ICD11:
XN3NM 
]
The structure was elucidated in this paperNCBI PubMed ID: 8548782Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Laboratory of Bacteriology and Medical Mycology, Instituto Supeiore di Sanita, Rome, Italy
The type VII capsular polysaccharide isolated from the newly discovered group B Streptococcus (GBS) strain contains D-glucose, D-galactose, N-acetyl-D-glucosamine and N-acetylneuraminic acid in the molar ratio 2:2:1:1. High-resolution one- and two-dimensional (1D and 2D) 1H and 13C NMR spectroscopy of the native and desialylated polysaccharides showed the type VII GBS capsular polysaccharide to contain the following branched hexasaccharide repeating unit: [formula: see text] Despite extensive structural similarity with the previously described GBS polysaccharides, the type VII polysaccharide showed no cross-reaction with the heterologous antisera.
group B Streptococcus, NMR spectroscopy, Type VII capsular polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: page 4
Compound class: CPS
Contained glycoepitopes: IEDB_130646,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_140108,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_30,SB_6,SB_7,SB_88
Methods: NMR, desialylation
Related record ID(s): 2843
NCBI Taxonomy refs (TaxIDs): 1319Reference(s) to other database(s): GTC:G11952DL, GlycomeDB:
25882
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There is only one chemically distinct structure:
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Paoletti LC, Johnson KD
Purification of preparative quantities of group B Streptococcus type III oligosaccharides
Journal of Chromatography A 705 (1995)
363-368
a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+
|
-4)-b-D-Glcp-(1-6)-b-D-GlcpNAc-(1-3)-b-D-Galp-(1- |
Show graphically |
Streptococcus sp. B III
(Ancestor NCBI TaxID 1319,
species name lookup)
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus [ICD11:
XN3NM 
]
NCBI PubMed ID: 7640771Journal NLM ID: 9318488Publisher: Amsterdam; New York: Elsevier
Institutions: Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Many bacterial capsular polysaccharides are regularly repeating units of oligosaccharides. Bacterial oligosaccharides have been used in neoglycoconjugate vaccines and as reagents in the study of specific antibody binding. Unfortunately, separation methods have not been adequate for the purification of preparative quantities of bacterial oligosaccharides. Here we describe a size-exclusion procedure that resulted in the resolution of group B Streptococcus type III oligosaccharides composed of 4-25 sugars.
polysaccharide, Streptococcus, Oligosaccharides, type, group B Streptococcus, preparation, preparative, purification
Structure type: polymer chemical repeating unit
Location inside paper: fig. 1
Trivial name: PS-III
Compound class: EPS, CPS, polysaccharide
Contained glycoepitopes: IEDB_1083493,IEDB_1083495,IEDB_130646,IEDB_130697,IEDB_135813,IEDB_136044,IEDB_136794,IEDB_137340,IEDB_137472,IEDB_137776,IEDB_1391966,IEDB_1392542,IEDB_140108,IEDB_140110,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142344,IEDB_142351,IEDB_142487,IEDB_142488,IEDB_143634,IEDB_146100,IEDB_146107,IEDB_146664,IEDB_149138,IEDB_149139,IEDB_149141,IEDB_149142,IEDB_149143,IEDB_149144,IEDB_149145,IEDB_149147,IEDB_149148,IEDB_149150,IEDB_149151,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_161524,IEDB_190606,IEDB_423120,IEDB_983931,SB_115,SB_116,SB_131,SB_145,SB_165,SB_166,SB_170,SB_171,SB_172,SB_173,SB_187,SB_192,SB_195,SB_30,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
Methods: 1H NMR, TLC, HPSEC, desialylation, FPLC
Biological activity: Oligosaccharides of group B Streptococcus (GBS) type III have been used in the design of neoglycoconjugates vaccines
Comments, role: Oligosaccharides composed of 4-25 monosaccharide residues.
Related record ID(s): 7852
NCBI Taxonomy refs (TaxIDs): 1319Reference(s) to other database(s): GTC:G35073UM, GlycomeDB:
11408, CCSD:
43274, CBank-STR:17387
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There is only one chemically distinct structure:
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Paoletti LC, Johnson KD
Purification of preparative quantities of group B Streptococcus type III oligosaccharides
Journal of Chromatography A 705 (1995)
363-368
b-D-Galp-(1-4)-+
|
-4)-b-D-Glcp-(1-6)-b-D-GlcpNAc-(1-3)-b-D-Galp-(1- |
Show graphically |
Streptococcus sp. B III
(Ancestor NCBI TaxID 1319,
species name lookup)
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus [ICD11:
XN3NM 
]
NCBI PubMed ID: 7640771Journal NLM ID: 9318488Publisher: Amsterdam; New York: Elsevier
Institutions: Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Many bacterial capsular polysaccharides are regularly repeating units of oligosaccharides. Bacterial oligosaccharides have been used in neoglycoconjugate vaccines and as reagents in the study of specific antibody binding. Unfortunately, separation methods have not been adequate for the purification of preparative quantities of bacterial oligosaccharides. Here we describe a size-exclusion procedure that resulted in the resolution of group B Streptococcus type III oligosaccharides composed of 4-25 sugars.
polysaccharide, Streptococcus, Oligosaccharides, type, group B Streptococcus, preparation, preparative, purification
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1
Compound class: O-polysaccharide, O-antigen, CPS
Contained glycoepitopes: IEDB_130646,IEDB_130697,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137776,IEDB_1391966,IEDB_1392542,IEDB_140108,IEDB_140110,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142351,IEDB_142487,IEDB_142488,IEDB_143634,IEDB_146664,IEDB_149138,IEDB_149139,IEDB_149141,IEDB_149142,IEDB_149143,IEDB_149144,IEDB_149145,IEDB_149147,IEDB_149148,IEDB_149150,IEDB_149151,IEDB_151531,IEDB_190606,IEDB_983931,SB_145,SB_165,SB_166,SB_173,SB_187,SB_192,SB_195,SB_30,SB_6,SB_7,SB_88
Methods: 1H NMR, TLC, HPSEC, desialylation, FPLC
Biological activity: Oligosaccharides of group B Streptococcus (GBS) type III have been used in the design of neoglycoconjugates vaccines
Comments, role: desialylated capsular polysaccharide; Oligosaccharides composed of 4-25 monosaccharide residues.
Related record ID(s): 7792
NCBI Taxonomy refs (TaxIDs): 1319Reference(s) to other database(s): GTC:G52755VD, GlycomeDB:
2039, CCSD:
11172, CBank-STR:12723
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There is only one chemically distinct structure:
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