A highly potent allelopathic factor, lepidimoide, was initially extracted from mucilage of germinated cress seeds. Polysaccharide extracted from okra (Abelmoschus esculentum Moench) is considered to have a similar structure to lepidimoide as its repeating unit. We therefore initiated the screening of enzymes capable of degrading okra polysaccharide into lepidimoide from endophytes. We discovered an endophytic fungal strain AHU9748 isolated from Coleus galeatus, which produced an oligosaccharide having similar properties to lepidimoide on thin layer chromatography. The physico-chemical data from ESI-MS, NMR spectra and other analyses also showed the purified product to be identical to lepidimoide. The strain AHU9748 was identified as a fungus belonging to the coelomycetes, closely related to the genus Colletotrichum, based on morphological characteristics and sequence analysis of the 18S rDNA and ITS region
endophytic fungi, lepidimoide, polysaccharide from okra, microbial degradation, Colletotrichum sp.
NCBI PubMed ID: 16233245Publication DOI: 10.1016/s1389-1723(02)80233-xJournal NLM ID: 100888800Publisher: Osaka, Japan, Amsterdam, The Netherlands: Society for Bioscience and Bioengineering
Correspondence: ftomita@chem.agr.hokudai.ac.jp
Institutions: Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan, Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan, R & D Center for Biotechnology, LIPI, Bogor, Indonesia
Methods: 13C NMR, 1H NMR, IR, DNA sequencing, TLC, ESI-MS, HPLC, extraction, CC, cell growth, precipitation, centrifugation
Found