From the underground parts of Ruscus aculeatus, a new bisdesmosidic spirostanol saponin named aculeosides B (2) was isolated, and its structure was determined on the basis of spectroscopic analysis, including 2D NMR techniques. Aculeoside A (1), which was previously isolated from the same plant source, exhibited inhibitory activity on cell growth of leukemia HL-60 cells with an IC50 value of 0.48 microgram mL(-1), while aculeoside B (2) was inactive.
NMR spectroscopy
NCBI PubMed ID: 9784168Publication DOI: 10.1021/np9704563Journal NLM ID: 7906882Publisher: American Society of Pharmacognosy
Correspondence: Mimaki Y
Institutions: School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
Methods: 13C NMR, 1H NMR, NMR-2D, IR, FAB-MS, TLC, acid hydrolysis, HPLC, optical rotation measurement, NaBH4 reduction, CC, HMBC, COSY, MTT, HOHAHA, HR-FAB-MS, extraciton, cytostatic activity assay
Found