Biotransformation of 1 mM (E)-cinnamic acid by thirty-day-old whole ribworts (Plantago lanceolala) was investigated. After addition of this caffeic acid precursor in medium culture, the root accumulation of the two main caifeic acid glycoside esters (CGEs), plantamoside and verbascoside was compared with in vitro ribwort control. No major modification of these two CGEs storage was highlighted, p-Coumaroyl-glucose and feruloyl-glucose were neo-synthesized and accumulated in the roots of seedlings fed with cinnamic acid. Maximum level of p-coumaroyl-glucose was higher than those produced by seventy-five-day-old ribworts previously investigated. Hence, the complete structure of the latter glucosylated acid was determined by both H-1 and C-13 NMR spectroscopy as (E)-p-coumaroyl-1-O-beta-D-glucopyranoside.
biotransformation, verbascoside, Plantago lanceolata, (E)-cinnamic acid, plantamoside
Publication DOI: 10.1007/BF02877824Journal NLM ID: 100955707Publisher: La Société
Institutions: Laboratoire de Botanique, Phytochimie et Mycologie, Faculté de Pharmacie, Montpellier, France, Laboratoire de Chimie Thérapeutique, Faculté de Pharmacie, Tours, France
Methods: 13C NMR, 1H NMR, TLC, HPLC, extraction, LC/MS
Found