Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 22724405Publication DOI: 10.1111/j.1574-695X.2012.01000.xJournal NLM ID: 9315554Publisher: Elsevier
Correspondence: knirel
ioc.ac.ru
Institutions: N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Shigella flexneri is an important human pathogen causing shigellosis. Strains of S. flexneri are serologically heterogeneous and, based on O-antigens, are currently classified into 14 types. Structures of the O-antigens (O-polysaccharides) of S. flexneri have been under study since 1960s but some gaps still remained. In this work, using one- and two-dimensional (1) H- and (13) C-NMR spectroscopy, the O-polysaccharides of several S. flexneri types were reinvestigated, and their structures were either confirmed (types 2b, 3b, 3c, 5b, X) or amended in respect to the O-acetylation pattern (types 3a, Y, 6, 6a). As a result, the O-acetylation sites were defined in all O-polysaccharides that had not been studied in detail earlier, and the long story of S. flexneri type strain O-antigen structure elucidation is thus completed. New and published data on the S. flexneri O-antigen structures are summarized and discussed in view of serological and genetic relationships of the O-antigens within the Shigella group and between S. flexneri and Escherichia coli.
Shigella flexneri, O-acetylation, serological classification, O-Polysaccharide structure, O-antigen diversity
Structure type: suggested polymer biological repeating unit
Location inside paper: p.206, table 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_125613,IEDB_125614,IEDB_127514,IEDB_133752,IEDB_133753,IEDB_133754,IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_141815,IEDB_141816,IEDB_142488,IEDB_143253,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_153213,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, ELISA, de-O-acetylation, NMR-1D, GPC
Comments, role: The O-antigen is structurally similar and serologically related to that of S. flexneri type 2a
Related record ID(s): 329, 11565, 25391, 27278, 28558, 28559, 28560, 28561, 28562, 28563, 28564, 28565, 28566, 28567, 28568, 28569, 28570, 28571, 28572, 28573, 28869, 30445
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G79469FG
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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