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Structure type: oligomer
Trivial name: core-lipid A carbohydrate backbone
Contained glycoepitopes: IEDB_130650,IEDB_135394,IEDB_137340,IEDB_137777,IEDB_140956,IEDB_141807,IEDB_151531

• Article ID: 1027
  Müller-Loennies S, Brade L, Brade H "Chemical structure and immunoreactivity of the lipopolysaccharide of the deep rough mutant I-69 Rd--/b+ of Haemophilus influenzae" - European Journal of Biochemistry 269(4) (2002) 1237-1242
  

  From the lipopolysaccharide of the deep rough mutant I-69 Rd--/b+ of Haemophilus influenzae two oligosaccharides were obtained after de-O-acylation and separation by high-performance anion exchange chromatography. Theirblankchemical structures were determined by one- and two-dimensional 1H-, 13C- and 31P-NMR spectroscopy as αKdo-4P-(2→6)-βGlcN-4P-(1→6)-αGlcN-1P and αKdo-5P-(2→6)-βGlcN-4P-(1→6)-αGlcN-1P. The specificity of mAbs S42-21 and S42-16 specific for Kdo-4P or Kdo- 5P, respectively [Rozalski, A., Brade L., Kosma P., Moxon R., Kusumoto S., & Brade H. (1997). Mol. Microbiol. 23, 569-577] was confirmed with neoglycoconjugates obtained by conjugation of the isolated oligosaccharides to BSA. In addition, a mAb S42-10-8 with unknown epitope specificity could be assigned using the neoglycoconjugates described herein. This mAb binds to an epitope composed of the bisphosphorylated glucosamine backbone of lipid A and Kdo-4P, whereby the latter determines the specificity strictly by the position of the phosphate group

  Lipopolysaccharide, Haemophilus, Haemophilus influenzae, oligosaccharide, structure, group, Research, Kdo, epitope, lipid, lipid A, phosphate, Oligosaccharides, MAb, specific, mutant, specificity, chemical, chemical structure, position, medicine, spectroscopy, backbone, rough, chromatography, separation, exchange, Glucosamine, acylation, two-dimensional, epitope specificity, anion-exchange, BSA, anion, immunoreactivity, neoglycoconjugate

  NCBI PubMed ID: 11856357
  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Correspondence: hbrade@fz-borstel.de
  Institutions: Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
  Methods: serological methods
  
   
  
  Haemophilus influenzae mutant I-69 (Rd--/b+)
  CSDB ID 3722 (all data & tools)
• Article ID: 1385
  Brabetz W, Müller-Loennies S, Brade H "3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase (WaaA) and Kdo kinase (KdkA) of Haemophilus influenzae are both required to complement a waaA knockout mutation of Escherichia coli" - Journal of Biological Chemistry 275(45) (2000) 34954-34962
  

  The lipopolysaccharide (LPS) of the deep rough mutant Haemophilus influenzae I69 consists of lipid A and a single 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) residue substituted with one phosphate at position 4 or 5 (Helander, I. M., Lindner, B., Brade, H., Altmann, K., Lindberg, A. A., Rietschel, E. T., and Zähringer, U. (1988) Eur. J. Biochem. 177, 483-492). The waaA gene encoding the essential LPS-specific Kdo transferase was cloned from this strain, and its nucleotide sequence was identical to H. influenzae DSM11121. The gene was expressed in the Gram-positive host Corynebacterium glutamicum and characterized in vitro to encode a monofunctional Kdo transferase. waaA of H. influenzae could not complement a knockout mutation in the corresponding gene of an Re-type Escherichia coli strain. However, complementation was possible by coexpressing the recombinant waaA together with the LPS-specific Kdo kinase gene (kdkA) of H. influenzae DSM11121 or I69, respectively. The sequences of both kdkA genes were determined and differed in 25 nucleotides, giving rise to six amino acid exchanges between the deduced proteins. Both E. coli strains which expressed waaA and kdkA from H. influenzae synthesized an LPS containing a single Kdo residue that was exclusively phosphorylated at position 4. The structure was determined by nuclear magnetic resonance spectroscopy of deacylated LPS. Therefore, the reaction products of both cloned Kdo kinases represent only one of the two chemical structures synthesized by H. influenzae I69.

  transfer, Haemophilus influenzae, Escherichia coli, 3-deoxy-D-manno-oct-2-ulosonic acid, Kdo, transferase, lipid A, Legionella pneumophila, mutation

  NCBI PubMed ID: 10952982
  Publication DOI: 10.1074/jbc.M005204200
  Journal NLM ID: 2985121R
  Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
  Correspondence: wbrabetz@fz-borstel.de
  Institutions: Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
  Methods: NMR
  
   
  
  Haemophilus influenzae 169, Escherichia coli WBB22
  CSDB ID 6285 (all data & tools)