The present study examines the effects of the extracts [petroleum ether, CHCl3, CHCl3/MeOH (9:1) and MeOH], partially purified fractions and pure compounds from Guettarda acreana on the electrically induced contractions (E.C.I.) of the isolated guinea-pig ileum. The results of the experiments indicate that CHCl3/MeOH (9:1), MeOH extract, and the MeOH soluble part from CHCl3/MeOH extract tested at concentrations of 1.2, 2.5, and 5 micrograms/ml, dose-dependently reduced the guinea-pig ileum contractions. Furthermore, some partially purified fractions I-IV from the MeOH extract, each tested at the same concentrations of the extracts, and some pure compounds (6 x 10(-6), 3 x 10(-6), 1 x 10(-6) M) isolated from the above fractions significantly reduced, in a dose-dependent manner, the electrical contractions of the ileum. The active compounds were identified as the known indole alkaloids strictosidic acid, lyalosidic acid, 5 alpha-carboxystrictosidine, strictosidine, and sickingine, as well as the known quinic acid derivatives 5-caffeoylquinic acid, 4,5-dicaffeoylquinic acid, and shikimic acid by spectral data. Two known quinovic acid glycosides and a new triterpenic glycoside, quinovic acid 3 beta-O-alpha-rhamnopyranosyl-(1-->3)-(beta-glucopyranosyl-(1-->6)-beta- glucopyranoside, were also isolated and their structures established by NMR and M5 data.
Rubiaceae, quinovic acid glycosides, indole alkaloids, Guettarda acreana, qunic acid derivatives, electrical contractions, guinea-pig ileum, antispasmodic
NCBI PubMed ID: 9619119Publication DOI: 10.1055/s-2006-957449Journal NLM ID: 0066751Publisher: George Thieme
Correspondence: Capasso A
Institutions: UMSA, Universidad Mayor de San Andres, Campus Universitario, La Paz, Bolivia, Dipartimento Scienze Farmaceutiche, Salerno, Italy, Dipartimento di Farmacologia Sperimentale, Università degli Studi di Napoli ''Federico II'', Napoli, Italy
Methods: 13C NMR, 1H NMR, FAB-MS, TLC, HPLC, extraction, optical rotation measurement, CC, biological assay, DEPT, COSY, HOHAHA, HETCOR, COLOC
Found