Bioactivity-guided fractionation of extracts of the aerial parts of Jasminum azoricum var. travancorense, using an in vitro ACE inhibition assay, led to isolation of three oligomeric, iridoid-type compounds, which were named sambacein I-III. Their structures are based on spectroscopic and chemical evidence. Similarly, fractionation of extracts of aerial parts of J. grandiflorum resulted in the isolation of the previously reported ACE inhibitor, oleacein. The IC50 values of purified ACE inhibitors were 26-36 microM. Moreover, 2-(3,4-dihydroxyphenyl)-ethanol, isoquercitrin and ursolic acid were isolated from J. grandiflorum. Sambaceins and oleacein are formed from genuine iridoid glucosides during processing of the plant material. NMR spectroscopy was used to measure the level of the ACE inhibitors in the traditional medicines prepared in Kerala from these Jasminum species.
NMR spectroscopy, isoquercitrin, ursolic acid, 2-(3, Oleaceae, Iridoids, oleuropein, Jasminum azoricum var. travancorense, J. grandiflorum, Indian traditional medicine, 4-dihydroxyphenyl)ethanol, molihuaside, oleacein, sambacein, sambacoside, C-13-NMR esterification shifts
NCBI PubMed ID: 9581523Publication DOI: 10.1055/s-2006-957419Journal NLM ID: 0066751Publisher: George Thieme
Correspondence: Vlietinck AJ
Institutions: Department of Medicinal Chemistry, The Royal Danish School of Pharmacy, Copenhagen, Denmark, Tropical Botanic Garden and Research Institute, Pacha-Palode, Trivandrum, Kerala, India, Carlsberg Laboratory, Department of Chemistry, Valby, Denmark, Department of Chemistry, Royal Veterinary and Agricultural University, Frederiksberg, Denmark
Methods: 13C NMR, 1H NMR, FAB-MS, TLC, extraction, CC, MPLC
Found