The structure of a neutral polysaccharide, isolated from the culture medium of Alcaligenes latus strain B-16, has been investigated by monosaccharide analysis, methylation analysis, one-(1D) and two-dimensional (2D) 1H and 13C NMR spectroscopy, and enzymatic digestion, and by MALDI-TOF-MS and LC-MS analyses of oligosaccharides isolated from partial acid hydrolysate. The polysaccharide has the linear disaccharide repeating unit. [formula: see text].
structural, structural studies, L-fucose, Alcaligenes, Alcaligenes latus, enzymatic hydrolysis, neutral polysaccharide, mannofucopyranan
Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Yokkaichi Laboratory, Hakuto Co., Ltd., 6-6-9, Betsumei, Yokkaichi, Mie 510, japan, Faculty of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Kyoto 606-01, Japan, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry, 1-1 Higashi Tsukuba, Ibaraki 305, Japan
Methods: methylation, NMR-2D, partial acid hydrolysis, NMR, MALDI-TOF MS, enzymatic digestion, LC-MS