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Structure type: polymer chemical repeating unit
Trivial name: acetan
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_136105,IEDB_140630,IEDB_141806,IEDB_142488,IEDB_144144,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72

• Article ID: 1067
  Ojinnaka C, Jay AJ, Colquhoun IJ, Brownsey GJ, Morris ER, Morris VJ "Structure and conformation of acetan polysaccharide" - International Journal of Biological Macromolecules 19 (1996) 149-156
  

  Acetan is an anionic bacterial polysaccharide. The chemical repeat unit consists of a cellobiose unit solubilised by attachment of a charged pentasaccharide sidechain to one of the glucose residues. The repeat unit contains two sites of acetylation. 1H and 13C NMR studies, coupled with both basic-methylation and mild-methylation studies, have shown that acetylation occurs at C6 on the (1,2)D-Man and the (1,34)D-Glc residues. A variety of techniques including NMR, optical rotation, circular dichroism and DSC show evidence for a thermoreversible conformational order (helix)-disorder (coil) transition for acetan in aqueous solution. The studies suggest that acetylation of the backbone does not prevent helix formation.

  NMR, conformation, structure, polysaccharide, acidic, methylation, extracellular, O-acetyl, acetan, localisation, A. xylinum polysaccharide, DSC, optical otation, circular dichroism

  NCBI PubMed ID: 8910054
  Journal NLM ID: 7909578
  Publisher: Butterworth-Heinemann
  Institutions: Department of Food Science and Technology, Cranfield University, Silsoe College, Silsoe, Bedford MK45 4DT, UK, IFR Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, UK
  Methods: 13C NMR, 1H NMR, methylation, differential scanning calorimetry (DSC), optical rotation measurement, CD
  
   
  
  Acetobacter xylinum B42
  CSDB ID 3792 (all data & tools)

Show legend Show as text

Structure type: polymer chemical repeating unit
Trivial name: acetan
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_136105,IEDB_140630,IEDB_141806,IEDB_142488,IEDB_144144,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72

• Article ID: 1067
  Ojinnaka C, Jay AJ, Colquhoun IJ, Brownsey GJ, Morris ER, Morris VJ "Structure and conformation of acetan polysaccharide" - International Journal of Biological Macromolecules 19 (1996) 149-156
  

  Acetan is an anionic bacterial polysaccharide. The chemical repeat unit consists of a cellobiose unit solubilised by attachment of a charged pentasaccharide sidechain to one of the glucose residues. The repeat unit contains two sites of acetylation. 1H and 13C NMR studies, coupled with both basic-methylation and mild-methylation studies, have shown that acetylation occurs at C6 on the (1,2)D-Man and the (1,34)D-Glc residues. A variety of techniques including NMR, optical rotation, circular dichroism and DSC show evidence for a thermoreversible conformational order (helix)-disorder (coil) transition for acetan in aqueous solution. The studies suggest that acetylation of the backbone does not prevent helix formation.

  NMR, conformation, structure, polysaccharide, acidic, methylation, extracellular, O-acetyl, acetan, localisation, A. xylinum polysaccharide, DSC, optical otation, circular dichroism

  NCBI PubMed ID: 8910054
  Journal NLM ID: 7909578
  Publisher: Butterworth-Heinemann
  Institutions: Department of Food Science and Technology, Cranfield University, Silsoe College, Silsoe, Bedford MK45 4DT, UK, IFR Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, UK
  Methods: 13C NMR, 1H NMR, methylation, differential scanning calorimetry (DSC), optical rotation measurement, CD
  
   
  
  Acetobacter xylinum B42
  CSDB ID 3861 (all data & tools)
• Article ID: 1893
  Kirby AR, Gunning AP, Morris VJ, Ridout MJ "Observation of the helical structure of the bacterial polysaccharide acetan by atomic force microscopy" - Biophysical Journal 68 (1995) 360-363
  

  A method has been developed that has been found to give reproducible images of uncoated polysaccharides by Atomic Force Microscopy (AFM). Aqueous solutions of the polysaccharide are deposited as drops onto freshly cleaved mica surfaces, air dried, and then imaged under butanol. The method has been used to obtain images of the bacterial polysaccharide acetan. In regions within the deposited sample, where the molecules are aligned side-by-side, it has been possible to observe a periodic structure along the polysaccharide chain, attributable to the helical structure of acetan.

  conformation, structure, Bacterial, polysaccharide, Bacterial polysaccharide, three-dimensional, acetan, helical, Microscopy, atomic force microscopy

  NCBI PubMed ID: 7711262
  Publication DOI: 10.1016/S0006-3495(95)80195-5
  Journal NLM ID: 0370626
  Publisher: Cambridge, MA: Cell Press
  Institutions: Institut of Food Research, Norwich Research Park, Colney, United Kingdom
  
   
  
  Acetobacter xylinum NRRL (B42)
  CSDB ID 111060 (all data & tools)
• Article ID: 2262
  Jansson PE, Lindberg J, Wimalasiri KMS, Dankert MA "Structural studies of acetan, an exopolysaccharide elaborated by Acetobacter xylinum" - Carbohydrate Research 245 (1993) 303-310
  

  The exopolysaccharide acetan, elaborated by Acetobacter xylinum, has been investigated. The polysaccharide and a heptasaccharide, obtained on enzymic hydrolysis, corresponding to the repeating unit were characterised by sugar and methylation analysis and by NMR spectroscopy and MS. It is concluded that the polysaccharide is composed of repeating units with the following structure. [formula: see text] The polysaccharide further contains approximately two O-acetyl groups per repeating unit, which have not been assigned, but it appears that they are on primary locations.

  NCBI PubMed ID: 8370027
  Publication DOI: 10.1016/0008-6215(93)80079-t
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden
  Methods: 13C NMR, 1H NMR, FAB-MS, enzymatic depolymerization
  
   
  
  Acetobacter xylinum
  CSDB ID 117293 (all data & tools)