Extracts from Bupleurum fruticescens were examined for oral and topical anti-inflammatory activities. The BuOH extract proved to be active against carrageenan and tetradecanoylphorbol acetate acute edemas and allowed the isolation of three saponins identified by spectroscopic techniques as 3β- O(O-α-L-rhamnopyranosyl-(1-→4)-O-[β-D-glucopyranosyl-(1 → 6)]O-β-D- glucopyranosyl)lup-20(29)-ene-23,28-diolc acid (fruticesaponin A), 3β-O-(O- α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl)lup-20(29)-ene-23,28-dioic acid 28-O-β-D-glucopyranosy(ester (fruticesaponin B), and 3βO-(O-α-L- rhamnopyranosyl-(1 → 4)-O-[β-D-glucopyra nosyl-(1 → 6)]-O-β-D- glucopyranosyl)lup-20(29)-ene-23,28-dioic acid 28-O-β-D-glucopyranosyl ester (fruticesaponin C). These compounds were studied against carrageenan, tetradecanoylphorbol acetate, arachidonic acid and ethyl phenylpropiolate acute edemas. Fruticesaponin B, a bi-desmosidic saponin with an unbranched saccharide moiety was the most active in all the tests applied.
Umbelliferae, Bupleurum fruticescens, in vivo anti-inflammatory activity (anti-edematous activity), lupane saponins
NCBI PubMed ID: 9690340Publication DOI: 10.1055/s-2006-957469Journal NLM ID: 0066751Publisher: George Thieme
Institutions: Dipartimento di Chimica Bioorganica, Universita di Pisa, Pisa, Italy, Department de Farmacologia, Universitat de València, Burjassot, Spain
Methods: biological assays
Found