The EtOH extract of the leaves of Holarrhena curtisii yielded five new steroidal alkaloids: 17-epiholacurtine (3), 17-epi-N-demethylholacurtine (4), holacurtinol (5), 3α-amino-14β-hydroxypregnan-20-one (7), and 15α- hydroxyholamine (8), in addition to the known compounds, holacurtine (1), N- demethylholacurtine (2); and holamine (6). All eight compounds showed significant cytotoxic and leishmanicidal activities.
Magnetic Resonance Spectroscopy, HL-60 cells, antiprotozoal agents, Holarrhena curtisii, leukemia P388
NCBI PubMed ID: 9834146Publication DOI: 10.1021/np970545fJournal NLM ID: 7906882Publisher: American Society of Pharmacognosy
Correspondence: Kam T
Institutions: Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia, Temko Corporation, Nakano, Tokyo, Japan, Kitasato Institute, Minato-ku, Tokyo, Japan
Methods: 13C NMR, 1H NMR, NOE, IR, extraction, optical rotation measurement, CC, HMBC, HMQC, COSY, HR-EI-MS
Found