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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115015,IEDB_1394181,IEDB_142489,IEDB_144831,IEDB_145005,IEDB_145006,IEDB_145010,IEDB_145011,IEDB_149135,SB_86

• Article ID: 1071
  Ovod V, Rudolph K, Knirel YA, Krohn K "Immunochemical characterization of O polysaccharides composing the a-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies" - Journal of Bacteriology 178 (1996) 6459-6465
  

  Murine monoclonal antibodies (MAbs) reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra- and tri-α-D-rhamnose repeats in the backbone [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1-2)D-Rha(α1] and [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1] were generated and used for immunochemical analysis and for serological classification of the bacteria. A total of 195 of 358 P. syringae strains tested representing 21 pathovars were shown to share a common epitope, 1a, and were classified into serogroup O1. All strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi, most of the strains with pathovars atrofaciens and striafaciens, and half of the strains with pathovar syringae were classified into serotypes O1a', O1b, O1c, and O1d within serogroup O1. Serogroup-specific epitope 1a was inferred to be related to the (α1-2)D-Rha(α1-3) site of the OPS backbone. The serotype-specific epitopes 1b, 1c, 1d, and 1a' were inferred as relating to the immunodominant lateral (α1-3)D-Rha, (β1-4)D-GlcNAc, and (α1-4)D-Fuc substituents and backbone-located site (α1-3)D-Rha(α1-2), respectively, of OPSs that share the common tetra-D-rhamnose repeats in the backbone. A total of 7.3% of the strains studied, all with pathovars morsprunorum and lapsa, were classified as serotypes O2a and O2d within serogroup 02. Serotype-specific epitope 2a was inferred as being related to the backbone-located site D-Rha(α1-3)D-Rha and epitope 2d to the immunodominant lateral (α1-4)D-Fuc residue of OPS consisting of tri-D-rhamnose repeats in the backbone. Epitope 2d alternated with 2a within the same LPS molecule and did not cross-react with epitope 1d. Serotypes O2a and O2d were observed in some strains correlating with the coexpression of the two chemotypes of OPS by the same strain. The serogroup O1-specific MAb Ps1a reacted weakly but definitely with all strains from serogroup 02. We propose serological formulas for serogroups O1 and 02 as well as for individual strains within these serogroups.

  Lipopolysaccharide, LPS, characterization, polysaccharide, polysaccharides, Pseudomonas, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, backbone, immunochemical, Pseudomonas syringae, classification, D-rhamnose

  NCBI PubMed ID: 8932301
  Journal NLM ID: 2985120R
  Publisher: American Society for Microbiology
  Correspondence: Ltvlov@uta.fi
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Department of Microbiology and Immunology, University of Kiev, Kiev, Ukraine, Institut fur Pflanzenpathologie und Pflanzenschutz der Georg-August-Universitat, Gottingen, Germany
  Methods: serological methods
  
   
  
  Pseudomonas syringae P-55
  CSDB ID 3872 (all data & tools)
• Article ID: 1073
  Ovod V, Knirel Y, Krohn K "Demonstration of the immunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and serotype-specific epitopes with monoclonal antibodies" - Proceedings of International Conference on Pseudomonas syringae Pathovars and Related Pathogens (5th : 1995 : Berlin, Germany) (1997) Vol. 9, 532-537
  

  Using serogroup- and serotyppe-specific murine monoclonal antibodies (MAbs) to Pseudomonas syringae lipopolysacharide (LPS) O-polysaccharides (OPS) (=O chains) with elucidated primary chemical structure of the O-repeating units, a rather high diversity of the OPS-related epitopes was demonstrated and most of them were inferred. The immunogenic properties of the O-serogroup- and O-serotype-specific epitopes were shown to depend on the nature and the number of sugar residues in the O-repeat as well as on the arrangement of the monosaccharides and the mode of linkages between them.

  Lipopolysaccharide, LPS, structure, strain, Pseudomonas, chain, group, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, epitopes, O-polysaccharide, serogroup, immunochemical, O-chain, pathogen, pathogens, pathovar, Pseudomonas syringae, classification, diversity, serotype-specific

  Publisher: Kluwer Academic Publishers, The Netherlands
  Correspondence: knirel@ioc.ac.ru
  Editors: Rudolph K, Burr TJ, Mansfield JW, Stead DE, Vivian A, von Kietzell J
  Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsy Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  
   
  
  Pseudomonas syringae pv. syringae P-55
  CSDB ID 4118 (all data & tools)
• Article ID: 2789
  Ovod V, Ashorn P, Yakovleva L, Krohn K "Classification of Pseudomonas syringae with monoclonal antibodies against the core and O-side chains of the lipopolysaccharide" - Phytopathology 85 (1995) 226-232
  
  Journal NLM ID: 9427222
  Publisher: American Phytopathological Society
  
   
  
  Pseudomonas syringae pv. syringae 218, Pseudomonas syringae pv. syringae P-55
  CSDB ID 146841 (all data & tools)