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1. (Article ID: 300)
 
Lagatolla C, Skerlavaj S, Dolzani L, Tonin EA, Monti BC, Bosco M, Rizzo R, Giglio L, Cescutti P
Microbiological characterisation of Burkholderia cepacia isolates from cystic fibrosis patients: investigation of the exopolysaccharides produced
FEMS Microbiology Letters 209(1) (2002) 95-102
 

Eleven strains of Burkholderia cepacia were isolated directly from clinical specimens: 10 from sputum of cystic fibrosis patients, and one from a vaginal swab. They were biochemically identified using API20NE and confirmed by a PCR-based assay. The genomovar characterisation obtained by specific PCR amplification revealed seven strains belonging to genomovar I, three belonging to genomovar IIIA and one belonging to genomovar IV. All isolates were also typed by ribotyping and random amplification of polymorphic DNA analysis. Some of the characterised strains were examined for the ability to produce exopolysaccharides, with the aim of correlating the genomovar with the exopolysaccharide structure. The polysaccharides were analysed by means of methylation analysis and 1H NMR spectroscopy in order to determine structural similarities. It was shown that different strains are capable of producing chemically different polysaccharides.

Burkholderia, Burkholderia cepacia, Molecular Sequence Data, polysaccharides, adolescence, bacterial typing techniques, pneumonia

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2. (Article ID: 1620)
 
Herasimenka Y, Benincasa M, Mattiuzzo M, Cescutti P, Gennaro R, Rizzo R
Interaction of antimicrobial peptides with bacterial polysaccharides from lung pathogens
Peptides 26(7) (2005) 1127-1132
 

The interaction of two cathelicidin antimicrobial peptides, LL-37 and SMAP-29, with three bacterial polysaccharides, respectively, produced by Pseudomonas aeruginosa, Burkholderia cepacia and Klebsiella pneumoniae, was investigated to identify possible mechanisms adopted by lung pathogens to escape the action of innate immunity effectors. In vitro assays indicated that the antibacterial activity of both peptides was inhibited to a variable extent by the three polysaccharides. Circular dichroism experiments showed that these induced an alpha-helical conformation in the two peptides, with the polysaccharides from K. pneumoniae and B. cepacia showing, respectively, the highest and the lowest effect. Fluorescence measurements also indicated the presence of peptide-polysaccharide interactions. A model is proposed in which the binding of peptides to the polysaccharide molecules induces, at low polysaccharide to peptide ratios, a higher order of aggregation, due to peptide-peptide interactions. Overall, these results suggest that binding of the peptides by the polysaccharides produced by lung pathogens can contribute to the impairment of peptide-based innate defenses of airway surface

conformation, chemistry, Bacterial, microbiology, pathogenicity, Non-U.S.Gov't, polysaccharide, Burkholderia, Burkholderia cepacia, Pseudomonas, Pseudomonas aeruginosa, molecule, antigens, Carbohydrate Sequence, induced, Molecular Sequence Data, Klebsiella, Bacterial polysaccharide, activity, polysaccharides, mechanism, biochemistry, biophysics, surface, interaction, immunity, pathogen, pathogens, Klebsiella pneumoniae, bacterial polysaccharides, binding, measurement, action, effect, peptides, pneumonia, fluorescence, variable, assay, model, order, in vitro, defense, innate immunity, peptide, circular dichroism, pharmacology, macromolecular, Humans, Research Support, antimicrobial, aggregation, lung, antagonists & inhibitors, antibacterial, Antimicrobial Cationic Peptides, Blood Proteins

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