The three major flavonoids isolated from Arabidopsis thaliana plants grown in the greenhouse were identified by means of spectroscopic analysis (UV, NMR, MS) and chiral capillary zone electrophoresis as the novel kaempferol 3-O-β-[β-D-glucopyranosyl(1→6)D-glucopyranoside]-7-O-α-L-rhamnopyranoside (1), kaempferol 3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (2), and kaempferol 3-O-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside (3). Comprehensive NMR studies including selective 1D and gradient-enhanced 2D techniques were applied in order to achieve full signal assignment and definitive proof of linkage for compound 1.
flavonoid, Arabidopsis thaliana, flavone glycoside
NCBI PubMed ID: 10514319Publication DOI: 10.1021/np990080oJournal NLM ID: 7906882Publisher: American Society of Pharmacognosy
Correspondence: Pauli GF
Institutions: Department of Pharmaceutical Biology, University of Würzburg, Würzburg, Germany, Institute of Pharmaceutical Biology and Phytochemistry, Westfälische Wilhelms-University, Hittorfstrasse 56, D-48149 Münster, Germany
Methods: 13C NMR, 1H NMR, IR, TLC, MALDI-TOF MS, HPLC, UV, TOCSY, HMBC, capillary electrophoresis, COSY, HSQC, HRMS, TFA hydrolysis, reduction with NaCNBH3, API-MS
Found