Two saponins were isolated from the roots of Ardisia mamillata HANCE. Their structures were established on the basis of MALDI-TOFMS, 1H, 13C NMR and 2D NMR (COSY, HOHAHA, HETCOR, HMBC and ROESY) spectra, and on chemical evidence, to be ardisimamilloside A, 3-O-{α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-α-L-arabinopyranosyl}-3β, 16α,28α-trihydroxy-13β,28-epoxy-oleanan-30-al; and ardisimamilloside B, 3-O-{α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-α-L-arabinopyranosyl}-3β-hydroxy-13β,28-epoxy-oleanan-16-oxo-30-al.
triterpenoid saponin, Myrsinaceae, Ardisia mamillata, ardisimamilloside A, ardisimamilloside B
NCBI PubMed ID: 11014272Publication DOI: 10.1016/s0031-9422(00)00173-4Journal NLM ID: 0151434Publisher: Elsevier
Correspondence: takeda-td@kyoritsu-ph.ac.jp
Institutions: Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan, School of Pharmaceutical Sciences, West China University of Medical Sciences, Chengdu, China, Department of Pharmacognosy, Kyoritsu College of Pharmacy, Tokyo, Japan
Methods: 13C NMR, 1H NMR, NMR-2D, TLC, acid hydrolysis, GC, MALDI-TOF MS, HPLC, extraction, optical rotation measurement, acetylation, elemental analysis, reduction, CC, melting point determination, precipitation, derivatization, evaporation
Found