The structure was elucidated in this paper NCBI PubMed ID:22925767 Publication DOI:10.1016/j.carres.2012.05.018 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: G.M. Streshinskaya <streshinskayamail.ru> Institutions: School of Biology, M. V. Lomonosov Moscow State University, Moscow, Russian Federation, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, Russian Federation, All-Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia, Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Lviv 79005, Ukraine
The cell wall of Streptomyces coelicolor M145, a prototrophic plasmidless (SCP1(-) SCP2(-)) variant of strain S. coelicolor A3(2) contains the main glycopolymer represented by Kdn-containing teichulosonic acid with unusual structure which has not been described so far: [structure: see text]. The minor polymer was found to be a poly(diglycosyl 1-phosphate) with the following repeating unit: -6)-α-Galp-(1→6)-α-GlcpNAc-(1-P-. The structures of both glycopolymers were established by using a combination of chemical and NMR spectroscopic methods.
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, 31P NMR, acid hydrolysis, GLC, paper chromatography, NMR-1D, paper electrophoresis Comments, role: Part of the polymer I (see RR: 28627, 28628), where residue aKdnp may be without substituents.
Related record ID(s): 27312, 28625, 28626, 28627, 28628, 31083 NCBI Taxonomy refs (TaxIDs):1902 Reference(s) to other database(s): GTC:G70287XB Show glycosyltransferases
The structure was elucidated in this paper NCBI PubMed ID:22925767 Publication DOI:10.1016/j.carres.2012.05.018 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: G.M. Streshinskaya <streshinskayamail.ru> Institutions: School of Biology, M. V. Lomonosov Moscow State University, Moscow, Russian Federation, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, Russian Federation, All-Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia, Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Lviv 79005, Ukraine
The cell wall of Streptomyces coelicolor M145, a prototrophic plasmidless (SCP1(-) SCP2(-)) variant of strain S. coelicolor A3(2) contains the main glycopolymer represented by Kdn-containing teichulosonic acid with unusual structure which has not been described so far: [structure: see text]. The minor polymer was found to be a poly(diglycosyl 1-phosphate) with the following repeating unit: -6)-α-Galp-(1→6)-α-GlcpNAc-(1-P-. The structures of both glycopolymers were established by using a combination of chemical and NMR spectroscopic methods.
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, 31P NMR, acid hydrolysis, GLC, paper chromatography, NMR-1D, paper electrophoresis Comments, role: glycoform of the oligosaccharide from cell wall polysaccharide I.
Related record ID(s): 27312, 28625, 28626, 28627, 28628, 31082 NCBI Taxonomy refs (TaxIDs):1902 Reference(s) to other database(s): GTC:G60688AT Show glycosyltransferases